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Monarch®  RNA Cleanup Kit (50 µg)

Catalog # Concentration Size List Price Quantity Your Price
T2040L 100 preps $451.00
$405.90
T2040S 10 preps $90.00
$81.00
Catalog # Size List Price Your Price
T2040L 100 preps $451.00
$405.90
T2040S 10 preps $90.00
$81.00
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

The Monarch RNA Cleanup Kit (50 µg) enables fast and simple purification and concentration of up to 50 µg of RNA from enzymatic reactions.

  • Ideal for cleanup and concentration of RNA after enzymatic treatments including DNase I, Proteinase K, labeling, capping or in vitro transcription (IVT)
  • Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt)
  • Elute in ≥ 20 µl for concentrated RNA
  • 70-100% RNA recovery
  • Can be used to purify RNA from the aqueous phase following TRIzol® or similar extractions
  • Simplified workflow with a single wash buffer
  • Unique column design prevents buffer carryover and elution of silica particulates
  • Columns and buffers are available separately
  • Purified RNA is ready for use in a wide variety of downstream applications, including transfection

Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.

Featured Videos
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  • Monarch_MiniprepProtocol_VideoThumb
    Monarch® RNA Cleanup Protocol
  • Monarch_MiniprepProtocol_VideoThumb
    Tips for successful RNA Cleanup using the Monarch® RNA Cleanup Kits
  • Monarch_MiniprepProtocol_VideoThumb
    Avoiding RNase Contamination
  • Monarch_MiniprepProtocol_VideoThumb
    How to recycle your Monarch® Kit components

The Monarch RNA Cleanup Kit (50 µg) rapidly and reliably purifies up to 50 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design ensures zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 20 μl. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA library prep for NGS and RNA labeling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nts).

Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.

Monarch RNA Cleanup kits are also available for 10 µg (NEB #T2030) and 500 µg (NEB #T2050) binding capacities. Columns and buffers are also available separately for convenience.

 

Figure 1: Monarch RNA Cleanup Kit workflow

 

Specifications and Applications::

SPECIFICATIONS
RNA Sample Type Cleanup of previously purified RNA (TRIzol, phenol/ chloroform) and RNA from enzymatic reactions (in vitro transcription reactions, DNase I treatment)
Binding Capacity 50 µg
RNA Size Range ≥ 25 nt ( ≥ 15 nt with modified protocol)
Typical Recovery 70–100%
Elution Volume 20–50 µl
Purity A260/280 > 1.8 and A260/230 > 1.8
Protocol Time 5 minutes of spin and incubation time
Compatible Downstream Applications RT =-PCR, RNA library prep for NGS, Formation of ribonucleoprotein (RNP) complexes for genome editing studies, microinjection, RNA labeling, transfection

APPLICATIONS
RNA Cleanup and Concentration (including from the TRIzol aqueous phase) RNA purified by other methods can be further purified
Enzymatic Reaction Cleanup Enzymes such as RNA polymerases, DNase I, Proteinase K and phosphatases are removed allowing efficient desalting
In vitro Transcription Cleanup Enzymes and excess NTPs are removed to yield highly pure synthesized RNA
RNA Gel Extraction Purification of RNA from agarose gels
RNA Fractionation Fractionation of RNA into small and large RNA pools

 

Figure 2: The Monarch RNA Cleanup Kit enables efficient recovery of RNA in as little as 20 µl

rRNA (50 µg of 16S and 23S Ribosomal Standard from E. coli, Sigma) was purified using the Monarch RNA Cleanup Kit (50 µg, NEB #2040) and eluted with nuclease-free water using the elution volumes indicated. The percent recovery of RNA was calculated from the resulting A260, as measured using a Trinean DropSense™ 16. Approximately 80% of RNA can be efficiently recovered in as little as 20 µl.
Figure 3: The Monarch RNA Cleanup Kit (50 µg) produces sgRNA yields consistent with other competitor RNA cleanup kits and with lower residual NTP contamination

Six different sgRNA synthesis reactions from the EnGen® sgRNA Synthesis Kit, S. pyogenes (NEB #E3322) were cleaned up using either the Monarch RNA Cleanup Kit (50 µg, NEB #T2040) or a competitor kit (according to manufacturer’s recommendations) and eluted with 50 µl of nuclease-free water. sgRNA yield was calculated from the resulting A260, as measured using a Trinean Dropsense™ 16. The Monarch Cleanup Kit produced sgRNA yields consistent with other commercially available RNA cleanup kits.

Following cleanup, residual nucleotides (NTPs) were measured by LC-MS and are reported as (percent area NTPs (rATP+rCTP+rGTP+rUTP)/percent area sgRNA). The Monarch RNA Cleanup Kit consistently outperforms other commercially available RNA cleanup kits in the removal of residual NTPs from sgRNA synthesis reactions.  
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Monarch® RNA Cleanup Columns (50 μg) T2047-21 25 1 x 10 columns
  Monarch® Collection Tubes II T2018-21 25 1 x 10 tubes
  Monarch® Buffer BX T2041-21 25 1 x 3 ml
  Monarch® Buffer WX T2042-21 25 1 x 2.5 ml
  Nuclease-free Water B1500-21 25 1 x 2.5 ml
  Monarch® RNA Cleanup Columns (50 μg) T2047-1 25 2 x 50 columns
  Monarch® Collection Tubes II T2018-1 25 2 x 50 tubes
  Monarch® Buffer BX T2041-2 25 1 x 40 ml
  Monarch® Buffer WX T2042-2 25 2 x 20 ml
  Nuclease-free Water B1500-2 25 1 x 25 ml
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

FAQs
Troubleshooting
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Change Notifications

Effective March 25, 2024, component/product name changed to Monarch® Buffer BX. Product specifications have been updated.

Effective March 25, 2024, component/product name changed to Monarch® Buffer WX. Product specifications have been updated.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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