Item# Description List Price Web Price Qty
    T2047L Monarch® RNA Cleanup Columns (50 μg) - 100 columns $277.00
    $249.30
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    *On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

    Product Introduction

    The Monarch RNA Cleanup Columns (50 µg) are a component of the Monarch RNA Cleanup Kit (50 µg) (NEB #T2040) and can be used to purify up to 50 µg of RNA from enzymatic reactions. They are supplied with Monarch Collection Tubes II (NEB #T2018). RNA Cleanup Binding Buffer (NEB #T2041) and Monarch RNA Wash Buffer (NEB #T2042) are also available separately.

    • Ideal for the cleanup of RNA from enzymatic reactions including sgRNA synthesis, DNase I treatment, labeling, and capping.
    • Elute in ≥ 20 µl for concentrated RNA
    • 70-100% RNA recovery
    • Unique column design prevents buffer carryover and elution of silica particulates
    • Purified RNA is ready for use in a wide variety of downstream applications

    Product Information

    Description

    The Monarch RNA Cleanup Columns (50 µg) are a component of the Monarch RNA Cleanup Kit (50 µg) (NEB #T2040) and can be used to purify up to 50 µg of RNA from enzymatic reactions including DNase treatment (NEB #M0303), labeling, and capping. The columns are ideal for cleaning up RNA after in vitro transcription reactions such as sgRNA synthesis reactions (NEB #E3322). The columns are designed without the use of a retaining ring, ensuring no buffer retention and no carryover of contaminants. RNA can be eluted in as little as 20 μl for and is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and transfection.

    Monarch RNA Cleanup Columns are also available for 10 µg (NEB #T2037) and 500 µg (NEB #T2057) binding capacities.

    Figure 1: The Monarch RNA Cleanup Kit enables efficient recovery of RNA in as little as 20 µl

    rRNA (50 µg of 16S and 23S Ribosomal Standard from E. coli, Sigma) was purified using the Monarch RNA Cleanup Kit (50 µg, NEB #2040) and eluted with nuclease-free water using the elution volumes indicated. The percent recovery of RNA was calculated from the resulting A260, as measured using a Trinean DropSense 16. Approximately 80% of RNA can be efficiently recovered in as little as 6 µl.
    Figure 2: The Monarch RNA Cleanup Kit (50 µg) produces sgRNA yields consistent with other competitor RNA cleanup kits and with lower residual NTP contamination

    Six different sgRNA synthesis reactions from the EnGen® sgRNA Synthesis Kit, S. pyogenes (NEB #E3322) were cleaned up using either the Monarch RNA Cleanup Kit (50 µg, NEB #T2040) or a competitor kit (according to manufacturer’s recommendations) and eluted with 50 µl of nuclease-free water. sgRNA yield was calculated from the resulting A260, measured using a Trinean Dropsense 16. The Monarch Cleanup Kit produced sgRNA yields consistent with other commercially available RNA cleanup kits.

    Product Categories:
    RNA Cleanup Products,
    RNA Reagents Products,
    RNA Extraction & Purification Products
    ,
    Nucleic Acid Purification Products
    Applications:
    RNA Analysis,
    RNA Purification and Isolation

    Properties and Usage

      Storage Temperature

      25°C

      Faqs & Tech Tips

      FAQs

      1. What is the maximum binding capacity of the Monarch RNA Cleanup Column provided with the Monarch RNA Cleanup Kit?
      2. What is the smallest volume of nuclease-free water that can be used for elution with the Monarch RNA Cleanup Columns?
      3. Can I get better recovery with the Monarch RNA Cleanup Kits if I do a second elution with my eluent from the first elution?
      4. What factors affect my (A260/A230) when using the Monarch RNA Cleanup Kits?
      5. What size RNA can be purified with the Monarch RNA Cleanup Kit?
      6. Can I use the Monarch RNA Cleanup Kit to cleanup up my DNase I-treated RNA?
      7. Can I use the Monarch RNA Cleanup Kits to purify RNA from agarose gels?
      8. Can I use the Monarch RNA Cleanup Kits to cleanup RNA after a TRIzol®/chloroform extraction?
      9. Can I use the Monarch RNA Cleanup Kits to cleanup my in vitro transcription (IVT) reaction?
      10. Is the Monarch RNA Cleanup Kit (NEB #T2040) compatible with the EnGen sgRNA Synthesis Kit, S. pyogenes?
      11. Are the Monarch RNA Cleanup Kits compatible with Luna RT-qPCR reagents?
      12. Are the Monarch RNA Cleanup Kits compatible with NEBNext reagents for RNA library prep?
      13. How can I assess RNA integrity and purity?
      14. Are the columns in the Monarch RNA Cleanup Kits the same as those in the Monarch Total RNA Miniprep Kit (NEB #T2010)?
      15. Can I purchase Monarch® buffers and columns separately?

      Protocols & Manuals

      Manuals

      The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

      Protocols

      1. Monarch® RNA Cleanup Kit Protocol
      2. Purification of RNA from the Aqueous Phase Following TRIzol®/Chloroform Extraction using the Monarch® RNA Cleanup Kits
      3. Separation of Large and Small RNA into Fractions using the Monarch® RNA Cleanup Kits
      4. Extraction of RNA from Agarose Gels using the Monarch® RNA Cleanup Kits

      Other Tools & Resources

      Usage & Guidelines

      • Avoiding Ribonuclease Contamination
      • Guidelines for RNA Quantitation
      • Guidelines for Working with RNA During RNA Cleanup

      Troubleshooting Guides

      Troubleshooting Guide for RNA Cleanup

      Quality & Safety

      Quality Assurance Statement

      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

      Specifications

      The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

      Certificate Of Analysis

      The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]