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NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

Catalog # Concentration Size List Price Quantity Your Price
E7560S 96 reactions $7,765.00
$6,988.50
Catalog # Size List Price Your Price
E7560S 96 reactions $7,765.00
$6,988.50
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

The unique workflow of the NEBNext® Small RNA library prep kits addresses the challenge of minimization of adaptor-dimers while achieving production of high-yield, diverse multiplex libraries in a simple protocol.

  • Minimized adaptor-dimer contamination
  • High yields
  • Input RNA can be Total RNA
  • Suitable for methylated small RNAs (e.g., piRNAs) as well as unmethylated small RNAs
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The NEBNext Multiplex Small RNA Library Prep Kit for Illumina (Index Primers 1-48) contains the adaptors, primers, enzymes and buffers required to convert small RNAs into indexed libraries for next-generation sequencing on the Illumina platform. The novel workflow has been optimized to minimize adaptor-dimers, while producing high-yield, high-diversity libraries.

Each kit component must pass rigorous quality control standards, and each set of reagents is functionally validated together by construction and sequencing of indexed small RNA libraries on the Illumina sequencing platform.

 

NEBNext Multiplex Small RNA Library Prep Kit for Illumina (Index Primers 1-48)

E7560 workflow figure
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Gel Loading Dye, Blue E6138AAVIAL -20 1 x 1 ml 6 X
  NEBNext 3´ Ligation Enzyme Mix E7288AAVIAL -20 1 x 0.288 ml
  NEBNext Index 1 Primer for Illumina E7311AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 2 Primer for Illumina E7312AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 3 Primer for Illumina E7313AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 4 Primer for Illumina E7314AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 5 Primer for Illumina E7315AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 7 Primer for Illumina E7317AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 8 Primer for Illumina E7318AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 9 Primer for Illumina E7319AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 10 Primer for Illumina E7320AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 11 Primer for Illumina E7321AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 12 Primer for Illumina E7322AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 6 Primer for Illumina E7316AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext® Index 25 Primer for Illumina® E7561AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext® Index 27 Primer for Illumina® E7562AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext 3´ Ligation Reaction Buffer E7301AAVIAL -20 1 x 0.96 ml
  NEBNext 5´ Ligation Reaction Buffer E7304AAVIAL -20 1 x 0.096 ml
  NEBNext 5´ Ligation Enzyme Mix E7305AAVIAL -20 1 x 0.24 ml
  Murine RNase Inhibitor E7308AAVIAL -20 1 x 0.096 ml
  LongAmp™ Taq 2X Master Mix E7309AAVIAL -20 4 x 1.2 ml
  NEBNext SR Primer for Illumina E7310AAVIAL -20 1 x 0.24 ml
  DNA Gel Elution Buffer E7324AAVIAL -20 1 x 48 ml
  Linear Acrylamide E7325AAVIAL -20 1 x 0.192 ml
  TE Buffer E7326AAVIAL -20 1 x 1.92 ml
  Nuclease-free Water E7327AAVIAL -20 1 x 20 ml
  NEBNext 5´ SR Adaptor for Illumina E7328AVIAL -20 1 x 1,350 pmol
  NEBNext 3´ SR Adaptor for Illumina E7332AAVIAL -20 1 x 0.096 ml
  NEBNext SR RT Primer for Illumina E7333AAVIAL -20 1 x 0.096 ml
  NEBNext First Strand Synthesis Reaction Buffer E7334AAVIAL -20 1 x 0.768 ml
  ProtoScript II Reverse Transcriptase E7355AAVIAL -20 1 x 0.096 ml
  NEBNext Index 26 Primer for Illumina E7713AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 28 Primer for Illumina E7714AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 29 Primer for Illumina E7715AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 30 Primer for Illumina E7716AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 31 Primer for Illumina E7717AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 32 Primer for Illumina E7718AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 33 Primer for Illumina E7719AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 34 Primer for Illumina E7720AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 35 Primer for Illumina E7721AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 36 Primer for Illumina E7722AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 37 Primer for Illumina E7731AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 38 Primer for Illumina E7732AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 39 Primer for Illumina E7733AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 40 Primer for Illumina E7734AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 41 Primer for Illumina E7735AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 42 Primer for Illumina E7736AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 43 Primer for Illumina E7737AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 44 Primer for Illumina E7738AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 45 Primer for Illumina E7739AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 46 Primer for Illumina E7740AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 47 Primer for Illumina E7741AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 48 Primer for Illumina E7742AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 16 Primer for Illumina E7584AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 17 Primer for Illumina E7585AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 24 Primer for Illumina E7592AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 13 Primer for Illumina E7581AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 14 Primer for Illumina E7582AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 15 Primer for Illumina E7583AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 19 Primer for Illumina E7587AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 20 Primer for Illumina E7588AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 21 Primer for Illumina E7589AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 22 Primer for Illumina E7590AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 23 Primer for Illumina E7591AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 18 Primer for Illumina E7586AVIAL -20 1 x 0.01 ml 10 µM
  Quick-Load pBR322 DNA-MspI Digest E7323AAVIAL -20 1 x 0.96 ml

Properties & Usage

Materials Required but not Supplied

3 M Sodium Acetate, pH 5.5
100% Ethanol
80% Ethanol
Corning®, Costar®, Spin-X® Centrifuge Tube Filters (Cellulose Acetate Filters) (Sigma Aldrich #CLS8162)
Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)

Size Selection Materials:
    for gel size selection:
        6% Novex® TBE PAGE gel 1.0 mM
        10-well (Life Technologies, Inc. #EC6265BOX)
        SYBR® Gold Nucleic Acid Gel Stain (Life Technologies, Inc. #S-11494)
        RNase-free Disposable Pellet Pestles® (Kimble Kontes Asset Management, Inc.
        #749521-1590)
        Dry Ice/Methanol Bath or –80°C freezer

    for bead selection:
        Agencourt® AMPure® XP Beads (Beckman Coulter, Inc. #A63881)

    for Pippin Prep™ selection:
        3% Agarose Dye Free Gel (Sage Science #CDF 3010)

QIAquick® PCR Purification Kit (Qiagen® #28104)
Bioanalyzer® (Agilent® Technologies, Inc.)

Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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