1 kb Plus DNA Ladder

This product was previously named the 2-Log DNA Ladder. There have been no changes to product formulation or specifications. This product is available in Quick-Load Purple format - enjoy the convenience of a ready-to-load format.
Catalog # Concentration Size List Price Quantity Your Price
N3200L 1000 µg/ml 1000 gel lanes $365.00
$328.50
N3200S 1000 µg/ml 200 gel lanes $94.00
$84.60
Catalog # Size List Price Your Price
N3200L 1000 gel lanes $365.00
$328.50
N3200S 200 gel lanes $94.00
$84.60
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

A single convenient ladder for 100 bp up to 10 kb (100 bp and 1 kb ladder in one!)

  • Value pricing
  • Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
  • Easy to identify reference bands
  • Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes
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A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference points. The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.

Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.

NEB also offers a Quick-Load version of this ladder with purple dye.

  • Recommended gel percentage range: 1-1.4%
  • Optimum separation on 1.2% 
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  1 kb Plus DNA Ladder N3200SVIAL -20 1 x 0.1 ml 1 mg/ml
  Gel Loading Dye, Purple (6X), no SDS B7025SVIAL 25 1 x 1 ml 6 X

Properties & Usage

Bases

Fragment Mass (ng) bp
1 40 10002
2 40 8001
3 48 6001
4 40 5001
5 32 4001
6 120 3001
7 40 2017
8 57 1517
9 45 1200
10 122 1000
11 34 900
12 31 800
13 27 700
14 23 600
15 124 500/517
16 49 400
17 37 300
18 32 200
19 61 100

Effective Size Range

100bp to 10,002bp

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C



Materials Sold Separately
Notes
  • This ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
  • We recommend loading 0.5-1 μg of the 1 kb Plus DNA Ladder diluted in sample buffer.
  • All fragments have 4-base, 5´ overhangs that can be end labeled using T4 Polynucleotide Kinase (#M0201) or filled-in using DNA Polymerase I, Klenow Fragment (#M0210) (1). Use α-[32P] dATP or α-[32P] dTTP for the fill-in reaction.
  • 1 kb Plus DNA Ladder is stable for at least 3 months at 4°C.
  • For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH20.
  • 1X Gel Loading Dye, Purple (6X), no SDS:
    2.5% Ficoll®-400
    10mM EDTA
    3.3mM
    Tris-HCL (pH 8.0@25°C)
    0.02% Dye 1
    0.001% Dye 2
  • Due to the limitations of the acrylamide gel technology, one or two extra bands may be visible on the DNA ladders when run on a polyacrylamide gel.
References
  • Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual(2nd Ed.). 10.51-10.67.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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