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NEBNext® Ultra™ II FS DNA Module

Catalog # Concentration Size List Price Quantity Your Price
E7810L 96 reactions $1,508.00
$1,357.20
E7810S 24 reactions $419.00
$377.10
Catalog # Size List Price Your Price
E7810L 96 reactions $1,508.00
$1,357.20
E7810S 24 reactions $419.00
$377.10
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

This module is part of the NEBNext® Ultra™ II FS workflow, and is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595), for Illumina®-compatible library construction.

Kits that include reagents for every step in the Ultra II FS DNA library construction workflow are also available (NEBNext Ultra II FS DNA Library Prep Kit for Illumina NEB #E7805 and NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads NEB #E6177).

Note: The Ultra II FS (Fragmentation System) enzyme mix does not include NEBNext dsDNA Fragmentase®.




The NEBNext Ultra II FS DNA Module has been optimized to convert 100 pg - 0.5 μg of intact DNA to fragmented, end-repaired DNA having 5´ phosphorylated, 3´ dA-tailed ends. The module is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595), and is part of the Ultra II FS DNA workflow.

For Illumina library construction, the NEBNext Ultra II FS DNA Module is designed for use with the following:

For use with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) (NEB #E7395), refer to the Protocols tab for UMI Adaptors-specific guidance. 

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  NEBNext® Ultra™ II FS Enzyme Mix E7806AVIAL -20 1 x 0.048 ml
  NEBNext® Ultra™ II FS Reaction Buffer E7807AVIAL -20 1 x 0.168 ml
  TE Buffer (1X) E7808AVIAL -20 1 x 1.1 ml 1 X
  NEBNext® Ultra™ II FS Enzyme Mix E7806AAVIAL -20 1 x 0.192 ml
  NEBNext® Ultra™ II FS Reaction Buffer E7807AAVIAL -20 1 x 0.672 ml
  TE Buffer (1X) E7808AAVIAL -20 1 x 4.3 ml 1 X

Properties & Usage

Materials Required but not Supplied

Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)

Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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