New England Biolabs Canada
Interactive Tools

NEB Tools

NEBNext® Selector

Use this tool to guide you through selection of NEBNext reagents for next generation sequencing sample preparation.
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NEBcloner®

Use this tool to find the right products and protocols for each step (digestion, end modification, ligation and transformation) of your next traditional cloning experiment. Also, find other relevant tools and resources to enable protocol optimization. Click Here

NEBioCalculator®

Use this tool for your scientific calculations and conversions for DNA and RNA. Options include conversion of mass to moles, ligation amounts, conversion of OD to concentration, dilution and molarity. Click Here

NEBuilder®Assembly Tool

NEBuilder® Assembly Tool can be used to design primers for your Gibson Assembly reaction, based on the entered fragment sequences and the polymerase being used for amplification. Click Here

Enzyme Finder

Use this tool to select restriction enzymes by name, sequence, overhang or type. Enter your sequence using single letter code nomenclature, and Enzyme Finder will identify the right enzyme for the job. Click Here

DNA Sequences and Maps Tool

Use this tool to find the nucleotide sequence files for commonly used molecular biology tools, including plasmid, viral and bacteriophage vectors. Click Here

Double Digest Finder

Use this tool to guide your reaction buffer selection when setting up double-digests, a common timesaving procedure. Choosing the right buffers will help you to avoid star activity and loss of product. Click here

Competitor Cross-Reference Tool

Use this tool to select another company's competent cell product and find out which NEB strain is compatible. Choose either the product name or catalog number from the available selection, and this tool will identify the recommended NEB product and its advantages. A link to the product page where you can also order the product is provided. Click here

PCR Selection Tool

Use this tool to help select the right DNA polymerase for your PCR setup. Whether your amplicon is long, complex, GC-rich or present in a single copy, the PCR selection tool will identify the perfect DNA polymerase for your reaction. Click here

Tm Calculator

Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions. Click here

NEBcutter® V2.0

Use this tool to identify the restriction sites within your DNA sequence. Choose between Type II and commercially available Type III restriction enzymes to digest your DNA. NEBcutter® V2.0 will indicate cut frequency and methylation state sensitivity. Click here

Thermostable Ligase Reaction Temperature Calculator

This tool will estimate an optimal reaction temperature to minimize mismatch for thermostable ligation of two adjacent ssDNA probes annealed to a template. Click here

NEB Golden Gate Assembly Tool

Use this tool for your scientific calculations and conversions for DNA and RNA. Options include conversion of mass to moles, ligation amounts, conversion of OD to concentration, dilution and molarity. Click here

Additional Links

REBASE®

Use this tool as a guide to the ever-changing landscape of restriction enzymes. REBASE, the Restriction Enzyme DataBASE, is a dynamic, curated database of restriction enzymes and related proteins. Click here

Polbase®

Polbase is a repository of biochemical, genetic, and structural information about DNA Polymerases. Click here

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