Luna® Universal qPCR & RT-qPCR

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Fluorescence-based quantitative real-time PCR (qPCR) is the gold standard for the detection and quantification of nucleic acids due to its sensitivity and specificity. Luna products from NEB are optimized for qPCR or RT-qPCR, and are available for either intercalating dye or probe-based detection methods. All Luna products provide robust performance on diverse sample sources and target types.

Each Hot Start Taq-based Luna qPCR master mix has been formulated with a unique passive reference dye that is compatible across a wide variety of instrument platforms, including those that require a ROX reference signal. This means that no additional components are required to ensure machine compatibility. The mixes also contain dUTP, enabling carryover prevention when reactions are treated with NEB’s Antarctic Thermolabile UDG (NEB #M0372). A blue visible dye assists in tracking the reagents when pipetting into clear, multi-welled PCR plates. The reverse transcriptase, featured in the Luna RT-qPCR products is a novel, engineered WarmStart® enzyme developed for robust performance and increased thermostability. These features, combined with rapid, sensitive and precise real-time qPCR performance, make Luna the universal choice for all your qPCR and RT-qPCR experiments.

Make a simpler choice

  • One product per application simplifies selection
  • Convenient master mix formats and user-friendly protocols simplify reaction setup
  • Non-interfering, visible tracking dye helps to eliminate pipetting errors
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Experience best-in-class performance

  • All Luna products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
  • Products perform consistently across a wide variety of sample sources
  • A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products

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Optimize your RT-qPCR with Luna WarmStart® Reverse Transcriptase 

  • Novel, thermostable reverse transcriptase (RT) improves performance
  • WarmStart RT paired with Hot Start Taq increases reaction specificity and robustness

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Luna qPCR and RT-qPCRInterested in learning more about
Luna qPCR & RT-qPCR products?
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Available products include:

Luna Universal qPCR Master Mix
 
Rapid, sensitive, and precise dye-based qPCR detection and quantitation of target DNA and cDNA sequences 
 
Luna Universal Probe qPCR Master Mix
 
Rapid, sensitive, and precise probe-based qPCR detection and quantitation of target DNA and cDNA sequences
 
Luna Universal One-Step RT-qPCR Kit
 
Includes everything you need for rapid, sensitive, and precise dye-based qPCR detection and quantitation of RNA targets
Luna Universal Probe One-Step RT-qPCR Kit
 
Includes everything you need for rapid, sensitive, and precise probe-based qPCR detection and quantitation of RNA targets

 
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One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.