FAQ: What is the composition of each buffer provided with the Monarch® Spin Plasmid Miniprep Kit (NEB #T1110)?

The composition of the buffers is proprietary. We can, however, share the following:

  • Monarch Buffer B1 (Resuspension buffer): Tris-based resuspension buffer
  • Monarch Buffer B2 (Lysis buffer): Sodium hydroxide and SDS-based lysis buffer
  • Monarch Buffer B3 (Neutralization buffer): Guanidine hydrochloride-based neutralization buffer
  • Monarch Buffer BZ (Wash buffer 1): Guanidine/isopropanol-based wash buffer
  • Monarch Buffer WZ (Wash buffer 2): Ethanol-based wash buffer
  • Monarch Buffer EY (Elution buffer): 10 mM Tris, 0.1 mM EDTA, pH 8.5 elution buffer

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