FAQ: Before use, how should I prepare the buffers? Do I need to add isopropanol and/or ethanol to the buffers prior to use?
Yes, isopropanol and ethanol needs to be added to Monarch Buffer BZ and Monarch Buffer WZ, prior to use. Monarch RNase A should be added to Monarch Buffer B1 prior to use, and then the buffer should be stored at 4°C. Please see the Buffer Preparation Guidance (NEB #T1110) with specific instructions for the kits on preparing these buffers for use.