FAQ: What is the maximum binding capacity of the Monarch® Spin Columns S1A provided with the Monarch Spin PCR & DNA Cleanup Kit (5 μg) (NEB #T1130)?

The matrix supplied in each column can bind up to 5 μg of DNA. Purifying DNA from a PCR or other enzymatic reaction introduces components such as detergents, dyes, proteins, and nucleotides, all of which can compete for binding to the membrane and affect DNA recovery.
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