FAQ: What size primers can be effectively removed from a PCR reaction using the Monarch® Spin PCR & DNA Cleanup Kit (NEB #T1130)?

Primers up to 45 nucleotides in length are most efficiently removed using the standard conditions recommended in the kit. Longer primers may also be removed, but any secondary structure or complementarity to other DNA species in the mix may increase initial binding to the matrix and result in trace recovery.
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