Preparation of high quality libraries at high yield is a critical first step in next generation sequencing workflows. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA and RNA and target enrichment of DNA for next generation sequencing. NEBNext kits are carefully designed to improve yields and library diversity using a broad range of input amounts from a wide variety of sample types. This has been most recently expanded with the addition of a novel enzyme-based DNA fragmentation system in the NEBNext Ultra II FS DNA Library Prep Kit (NEB #E7805), which enables reliable high-yield high-quality library construction with as little as 100 pg of input DNA.
Another recent technology addition to the NEBNext product line is NEB #E7000) provides highly specific hybridization-based capture of 190 common cancer targets from 50 genes, while the NEBNext Direct BRCA1/BRCA2 Panel (NEB #E6627) generates 100% coverage of all protein coding regions in BRCA1 and BRCA2 genes.
- DNA Fragmentation
- DNA Library Preparation
- FFPE DNA Repair
- NEBNext FFPE DNA Repair Mix
- Illumina® Library Preparation
- DNA for Illumina®
- NEBNext® Ultra™ II DNA Library Prep
- NEBNext® Ultra™ II RNA Library Prep
- RNA for Illumina®
- Ion Torrent™ DNA Library Preparation
- DNA for Ion Torrent™
- NEBNext® Automation
- RNA Library Preparation
- Appendix A for use with NEBNext Ultra II RNA First Strand Synthesis Module (E7771)
- Appendix A for use with Ultra™ II RNA and Ultra II Directional RNA (E7760, E7765, E7770, E7775)
- Guidelines for Running Samples on the Illumina MiSeq (E7000)
- Guidelines for Setting up PCR Reactions (E6627X only)
- Guidelines for Setting Up PCR Reactions (E7000X only)
- Index Pooling Guidelines - 96 Single Index Kit (E6609)
- Protocol for a PCR reaction using NEBNext® Ultra™ II Q5® Master Mix (M0544)
- Protocol for Directional RNA-seq Workflows and NEBNext Ultra II RNA First Strand Synthesis Module (E7771)
- Protocol for NEBNext Direct BRCA1/BRCA2 Panel (E6627)
- Protocol for NEBNext Direct® Cancer HotSpot Panel (NEB #E7000)
- Protocol for Non-directional RNA-seq Workflows and NEBNext Ultra II RNA First Strand Synthesis Module (E7771)
- Protocol for use with FFPE RNA, NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, E7765)
- Protocol for use with FFPE RNA, NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (E6310) and NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770, E7775)
- Protocol for use with NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, E7765)
- Protocol for use with NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490) and NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770, E7775)
- Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (E6310) and NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770, E7775)
- Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310)
- Protocol for use with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (E7645, E7103)
- Protocol for use with NEBNext® Ultra™ II End Repair/dA-Tailing Module (E7546)
- Protocol for use with Purified mRNA or rRNA Depleted RNA
- Protocol for use with Purified mRNA or rRNA Depleted RNA and NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770, E7775)
- Protocol for use with rRNA Depleted FFPE RNA
- Protocol for use with rRNA Depleted FFPE RNA and NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770, E7775)
- Setting up the PCR Reactions
Addressing Challenges in Microbiome DNA Analysis
Improving Enzymatic DNA Fragmentation for Next Generation Sequencing Library Construction
The Quantitation Question: How does accurate library quantitation influence sequencing?
The determination of the number of sequencing-ready molecules present after library preparation is an important step in the next generation sequencing (NGS) workflow and has a strong influence on the success of both a sequencing run and a sequencing-based experiment.
- AGBT 2018 Wellcome Trust Sanger Institute poster: Thurston. S., et al. (2018). A Large Genome Centre Core Pipeline Refresh.
- Application of a Novel, Targeted Sequencing-Based Genotyping Approach for Cost Effective Marker Assessment in O. sativa (2019)
- Benefits of Illumina® Library Quantitation with NEBNext® Library Quant Kit (2015)
- Examining Sources of Error in PCR by Single-Molecule Sequencing (2017)
- High Quality Automation Libraries Prepared from a Broad Range of DNA Input Amounts (2017)
- High-Throughput Screening of 2300 Genetic Markers in S.lycopersicum using the NEBNext Direct Multiplexed Genotyping Approach (2019)
- NEBNext® Ultra™II FS DNA: A Robust Enzyme-based DNA Library Preparation Method Compatible with Plant and Animal Samples (2019)
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Learn more about the streamlined workflow for the NEBNext Ultra II Directional RNA Library Prep Kit.
This video walks you through the size selection and cleanup steps using the NEBNext Ultra II DNA
Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips.
Watch as Deyra explains how to optimize your RNA inputs for successful NGS library preparation.
View our tutorial for help visualizing NEBNext Direct’s unique and enabling workflow.
Learn about the innovative NEBNext Direct technology for target enrichment for next generation sequencing.
In this webinar, Laurence Etwiller and Jennifer Ong discuss their recent publications, and how library preparation affects sequencing accuracy and variant calling. View full Q & A summary.
Behind the paper: DNA damage is a pervasive cause of sequencing errors, directly confounding variant identification
NEB researchers published a paper in Science highlighting DNA damage as a prevalent source of errors in public cancer databases. Learn about how addressing this damage could improve the detection of low-frequency disease variants.
Target enrichment offers several advantages over more comprehensive genomic profiling for a variety of scientific and applications. During this webinar, we explore the advantages and limitations of target enrichment, and discuss how NEBNext Direct®, a novel solution for selective enrichment of genomic targets, addresses these challenges.
This video walks you through DNA Library Preparation using the NEBNext Ultra II DNA Library Prep Kit. The video also includes tips for optimization as well as safe stopping points.
Are you running a core sequencing facility? Watch as Eileen shares a few reasons that NEBNext products are particularly well suited to sequencing core labs.
These 12 quick tips for NGS library preparation are a great refresher if you're a seasoned pro, or a great introduction if you're a beginner.