ProPur_App_Hero

Protein Purification

Recombinant production of proteins is one of the most powerful techniques used in the Life Sciences. The ability to produce and purify a desired recombinant protein in high yield and purity permits a wide range of uses in industrial processes, diagnosis and treatment of diseases, and enabling basic and applied research.

At first glance, protein purification may appear to be a simple, straightforward process. Essentially, the gene of interest is expressed with an affinity tag, such as a polyhistidine tag, or fused to a protein, such as maltose binding protein (MBP). A crude lysate containing the overexpressed gene product is then passed over a specific resin with affinity to bind the tag or fused protein, while other proteins and contaminants are removed during a wash step. Lastly, the target protein is eluted from the affinity resin, resulting in the isolation of highly pure protein in appreciable yields.  However, not all proteins are or can be tagged, and different resins, beads or magnetic matrices may need to be used under different buffering conditions (such as varying pH and salt concentrations) to achieve optimal purity of the target protein. Thus, no single solution exists for successful purification of all recombinant proteins. Instead, it is beneficial to have access to a wide range of purification tools and a willingness to explore multiple approaches to better one’s chances for success.

 

Choose Type:

Protein Purification includes these areas of focus:
Affinity Purification & Expression Tags
His-tagged Protein Expression & Purification
IMPACT Affinity Tag
MBP Affinity Tag
Fusion Protein Cleavage
FAQs for Protein Purification
Protocols for Protein Purification
Legal Information
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.