FAQ: What size of DNA can be purified with the Monarch DNA Cleanup Columns?
Recovery of DNA from 50 bp to 25 kb can be accomplished with the Monarch
DNA Cleanup Columns, supplied in both the DNA Gel Extraction Kit and
the PCR & DNA Cleanup Kit (5 µg). Efficiency decreases with longer
DNA due to tighter binding to the matrix. We observe higher recoveries
for DNA between 50 bp-5 kb than for DNA between 5-10 kb. Longer DNA will
also be recovered with reduced efficiencies. For optimal recovery of
large DNA fragments, please follow the instructions in the manual, which
include heating the elution buffer to 50°C prior to elution.
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