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DNA Modifying Enzymes

NEB offers a comprehensive list of tools and reagents for DNA manipulation. These include ligases, nucleases, methyltransferases, DNA repair proteins, recombinases and more.

NEBcloner is a guide for selecting appropriate products and viewing protocols for steps in the cloning workflow.

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DNA Modifying Enzymes and Cloning Technologies

5-hydroxymethyluridine DNA Kinase

Nucleoside Digestion Mix

Thermolabile Exonuclease I

DNA Ligases

9°N™ DNA Ligase

Blunt/TA Ligase Master Mix

E. coli DNA Ligase

ElectroLigase®

HiFi Taq DNA Ligase

Instant Sticky-end Ligase Master Mix

Quick Blunting™ and Quick Ligation™ Kits

Quick Ligation™ Kit

SplintR® Ligase

T3 DNA Ligase

T4 DNA Ligase

T4 DNA Ligase Reaction Buffer

T7 DNA Ligase

Taq DNA Ligase

Taq DNA Ligase Reaction Buffer

Thermostable 5´ App DNA/RNA Ligase

DNA Repair Proteins

Afu Uracil-DNA Glycosylase (UDG)

Antarctic Thermolabile UDG

APE 1

Endonuclease III (Nth)

Endonuclease IV

Endonuclease V

Endonuclease VIII

EnGen® Mutation Detection Kit

Fpg

hAAG

hSMUG1

PreCR® Repair Mix

RecA

RecAf

T4 PDG (T4 Endonuclease V)

T7 Endonuclease I

Thermolabile USER® II Enzyme

Tma Endonuclease III

Topoisomerase I (E. coli)

Tth Endonuclease IV

Uracil Glycosylase Inhibitor (UGI)

Uracil-DNA Glycosylase (UDG)

USER® Enzyme

Methyltransferases

AluI Methyltransferase

BamHI Methyltransferase

CpG Methyltransferase (M.SssI)

dam Methyltransferase

EcoGII Methyltransferase

EcoRI Methyltransferase

GpC Methyltransferase (M.CviPI)

HaeIII Methyltransferase

Hhal Methyltransferase

HpaII Methyltransferase

Human DNA (cytosine-5) Methyltransferase (Dnmt1)

MspI Methyltransferase

TaqI Methyltransferase

Nucleases

Afu Uracil-DNA Glycosylase (UDG)

Antarctic Thermolabile UDG

APE 1

Cas9 Nuclease NLS, S. pyogenes

Cas9 Nuclease, S. pyogenes

DNase I (RNase-free)

DNase I Reaction Buffer

Endonuclease III (Nth)

Endonuclease IV

Endonuclease V

Endonuclease VIII

EnGen® Mutation Detection Kit

EnGen® Spy Cas9 NLS

Exonuclease I (E. coli)

Exonuclease I Reaction Buffer

Exonuclease III (E. coli)

Exonuclease T

Exonuclease V (RecBCD)

Exonuclease VII

Exonuclease VIII, truncated

Fpg

hAAG

hSMUG1

Lambda Exonuclease

Micrococcal Nuclease

Mung Bean Nuclease

Nuclease BAL-31

Nuclease P1

Nucleoside Digestion Mix

PreCR® Repair Mix

RecAf

RecJf

T4 PDG (T4 Endonuclease V)

T5 Exonuclease

T7 Endonuclease I

T7 Exonuclease

Thermolabile Exonuclease I

Thermolabile USER® II Enzyme

Thermostable FEN1

Tma Endonuclease III

Tth Endonuclease IV

Uracil Glycosylase Inhibitor (UGI)

Uracil-DNA Glycosylase (UDG)

USER® Enzyme

Phosphatases & Sulfurylases

Alkaline Phosphatase, Calf Intestinal (CIP)

Antarctic Phosphatase

Antarctic Phosphatase Reaction Buffer

Pyrophosphatase, Inorganic (E. coli)

Pyrophosphatase, Inorganic (yeast)

Quick Dephosphorylation Kit

Shrimp Alkaline Phosphatase (rSAP)

Thermostable Inorganic Pyrophosphatase

Recombinases

Cre Recombinase

ssDNA Binding Proteins

ET SSB

RecA

RecAf

Kinases

T4 Polynucleotide Kinase

T4 Polynucleotide Kinase (3' phosphatase minus)

T4 Polynucleotide Kinase Reaction Buffer

DNA Labeling

DNA Polymerase I (E. coli)

Klenow Fragment (3'→5' exo-)

Streptavidin

T4 Polynucleotide Kinase

Terminal Transferase

Therminator™ DNA Polymerase

Other Products

TelN Protelomerase

β-Agarase I

DNA Ligases

9°N™ DNA Ligase

Blunt/TA Ligase Master Mix

E. coli DNA Ligase

ElectroLigase®

HiFi Taq DNA Ligase

Instant Sticky-end Ligase Master Mix

Quick Blunting™ and Quick Ligation™ Kits

Quick Ligation™ Kit

SplintR® Ligase

T3 DNA Ligase

T4 DNA Ligase

T4 DNA Ligase Reaction Buffer

T7 DNA Ligase

Taq DNA Ligase

Taq DNA Ligase Reaction Buffer

Thermostable 5´ App DNA/RNA Ligase

DNA Repair Proteins

Afu Uracil-DNA Glycosylase (UDG)

Antarctic Thermolabile UDG

APE 1

Endonuclease III (Nth)

Endonuclease IV

Endonuclease V

Endonuclease VIII

EnGen® Mutation Detection Kit

Fpg

hAAG

hSMUG1

PreCR® Repair Mix

RecA

RecAf

T4 PDG (T4 Endonuclease V)

T7 Endonuclease I

Thermolabile USER® II Enzyme

Tma Endonuclease III

Topoisomerase I (E. coli)

Tth Endonuclease IV

Uracil Glycosylase Inhibitor (UGI)

Uracil-DNA Glycosylase (UDG)

USER® Enzyme

Methyltransferases

AluI Methyltransferase

BamHI Methyltransferase

CpG Methyltransferase (M.SssI)

dam Methyltransferase

EcoGII Methyltransferase

EcoRI Methyltransferase

GpC Methyltransferase (M.CviPI)

HaeIII Methyltransferase

Hhal Methyltransferase

HpaII Methyltransferase

Human DNA (cytosine-5) Methyltransferase (Dnmt1)

MspI Methyltransferase

TaqI Methyltransferase

Nucleases

Afu Uracil-DNA Glycosylase (UDG)

Antarctic Thermolabile UDG

APE 1

Cas9 Nuclease NLS, S. pyogenes

Cas9 Nuclease, S. pyogenes

DNase I (RNase-free)

DNase I Reaction Buffer

Endonuclease III (Nth)

Endonuclease IV

Endonuclease V

Endonuclease VIII

EnGen® Mutation Detection Kit

EnGen® Spy Cas9 NLS

Exonuclease I (E. coli)

Exonuclease I Reaction Buffer

Exonuclease III (E. coli)

Exonuclease T

Exonuclease V (RecBCD)

Exonuclease VII

Exonuclease VIII, truncated

Fpg

hAAG

hSMUG1

Lambda Exonuclease

Micrococcal Nuclease

Mung Bean Nuclease

Nuclease BAL-31

Nuclease P1

Nucleoside Digestion Mix

PreCR® Repair Mix

RecAf

RecJf

T4 PDG (T4 Endonuclease V)

T5 Exonuclease

T7 Endonuclease I

T7 Exonuclease

Thermolabile Exonuclease I

Thermolabile USER® II Enzyme

Thermostable FEN1

Tma Endonuclease III

Tth Endonuclease IV

Uracil Glycosylase Inhibitor (UGI)

Uracil-DNA Glycosylase (UDG)

USER® Enzyme

Phosphatases & Sulfurylases

Alkaline Phosphatase, Calf Intestinal (CIP)

Antarctic Phosphatase

Antarctic Phosphatase Reaction Buffer

Pyrophosphatase, Inorganic (E. coli)

Pyrophosphatase, Inorganic (yeast)

Quick Dephosphorylation Kit

Shrimp Alkaline Phosphatase (rSAP)

Thermostable Inorganic Pyrophosphatase

Recombinases

Cre Recombinase

ssDNA Binding Proteins

ET SSB

RecA

RecAf

Kinases

T4 Polynucleotide Kinase

T4 Polynucleotide Kinase (3' phosphatase minus)

T4 Polynucleotide Kinase Reaction Buffer

DNA Labeling

DNA Polymerase I (E. coli)

Klenow Fragment (3'→5' exo-)

Streptavidin

T4 Polynucleotide Kinase

Terminal Transferase

Therminator™ DNA Polymerase

Other Products

TelN Protelomerase

β-Agarase I