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Competent Cells

Cloning Strains

For cloning experiments choose from several high efficiency competent cell strains. These E. coli strains are T1 phage resistant and are endA deficient for high quality plasmid preparations. Additionally, all competent cells from NEB are free of animal products.


NEB offers chemically competent Kluyveromyces lactis cells and variants of this strain that have been tailored for specific protein expression needs. These cells are suitable for transformation with any of our linearized pKLAC series expression vectors.

E. coli for Protein Expression

NEB offers several strains with varying levels of expression control, each with T1 phage resistance and extremely high transformation efficiencies.

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Competent Cells includes these subcategories:
Cloning Competent Cell Strains
E. coli Expression Strains Information
Yeast Expression Strains Information
FAQs for Competent Cells
Protocols for Competent Cells
Application Notes for Competent Cells
    Publications related to Competent Cells
  1. Narayanan, A., Ridilla, M. and Yernool, D.A. 2010. Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator–repressor interactions Pro. Sci. . 20 , PubMedID: , DOI:
  2. Schlegel, S., Klepsch, M., Gialama, D., Wickström, D., Slotboom, D.J. and de Gier, J. 2010. Revolutionizing membrane protein overexpression in bacteria  Micro. Biotech. . 3 , PubMedID: , DOI:
  3. Wagner, S., Klepsch, M., Schlegel, S., Appel, A., Draheim, R., Tarry, M., Hö, M., van Wijk, K.J., Slotboom, D.J., Persson, J.O. and de Gier, J. 2008. Tuning Escherichia coli for membrane protein overexpression PNAS . 105 , PubMedID: , DOI:
NEB Competent E. coli Strain Selection
NEB offers several stains with varying levels of expression control, each with phage T1 resistance and extremely high transformation efficiencies.