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NEB offers a number of endonucleases and exonucleases that cleave DNA.

Exonucleases catalyze the removal of nucleotides in either the 5’ to 3’ or the 3’ to 5’ direction from single or double stranded DNA. Endonucleases cleave DNA nonspecifically and can be used to generate blunt ends for ligation or to shorten duplex DNA.

Learn more about common applications and properties of exonucleases and endonucleases.


  1. Kunitz, M. (1950) J. Gen. Physiol. 33, 349-362. PMID: 15406373
  2. Vanecko, S. and laskowski, M. (1961) J. Biol. Chem. 236, 3312-3316. PMID: 13924728
  3. Cuatrecasas, S.F., and Anfinsen, C.B. (1967) J. Biol. Chem., 242, 1541-1547. PMID: 4290246

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Protocols for Nucleases
Application Notes for Nucleases
Common Applications of Exonucleases and Endonucleases
NEB provides a list of common applications for our exonucleases and endonucleases.
Properties of Exonucleases and Endonucleases
NEB supplies many nucleases; several characteristics should be considered when choosing the one best suited to your particular research needs.
Legal Information
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.