New England Biolabs (NEB) offers a selection of endoglycosidases, exoglycosidases, and heparinases for glycobiology research. Many of these reagents are recombinant, and all undergo several quality control assays, enabling us to provide products with lower unit cost, high purity and reduced lot-to-lot variation. All of our glycosidases are tested for contaminants. Since p-nitrophenyl-glycosides are not hydrolyzed by some exoglycosidases, we use only fluorescently-labeled oligosaccharides to screen for contaminating glycosidases.
To find all of our Glycobiology Application Notes, click here.
- How can I separate a deglycosylated protein from the glycosidase?
- What are the best techniques to analyze N- and O-glycans?
- What are the advantages of enzymatic deglycosylation?
- Do I need to deglycosylate my protein sample for proteomic analysis?
- Can I analyze protein glycosylation with only benchtop instrumentation and reagents?
- What are glycosidases and their uses?
- Can glycosidases be used in combination for extensive digestion?
N-Glycan Composition Profiling for Quality Testing of Biotherapeutics
The Structure, Function and Importance of Carbohydrates
Using Glycosidases to Remove Trim or Modify Glycans on Therapeutic Protein
The Glycoproteomics brochure provides information on the suite of endo- and exoglycosidases, and deglycosylation enzymes offered by NEB.
Glycoproteomics Technical Guide
Find in-depth information, including protocols, technical tips, frequently asked questions and application notes, utilizing NEB’s suite of endo- and exoglycosidases.
Rapid PNGase F Trifold
Learn how Rapid PNGase F can speed up your antibody deglycosylation step and make your therapeutic proteins faster and more efficiently than ever before.
- Improving Sample Workflow: Rapid PNGase F for Accurate Antibody Characterization (2015)
- Glycan Analyzer
- Endoglycosidase Selection Chart
- Detailed Characterization of Several Glycosidase Enzymes
- Glycobiology Unit Conversion Chart
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Learn about the core sequences and common modifications of N-linked and O-linked glycans in this video. Analysis of these glycans can be accomplished with the use of deglycosylation enzymes, which can provide complete sugar removal with no protein degradation.
Learn how glycosidases are used to analyze multiple glycoprotein samples. Here, the model glycoprotein, hCGβ, which carries both N- and O-glycans, is demonstrated.
Behind the Paper: An engineered Fbs1 carbohydrate binding protein for selective capture of N-glycans and N-glycopeptides
Minyong and Jim summarize their recent Nature Communications publication describing selective capture of N-glycans and N-glycopeptides by an engineered high affinity Fbs1 carbohydrate binding protein.
Learn more about the structural elucidation of complex and diverse heparin oligosaccharides using Bacteroides Heparinase I, II and III in combination with downstream mass spec analysis.
This one-step deglycosylation protocol is for use with New England Biolabs’ Rapid PNGase F. Learn more about Rapid PNGase F.
This two-step deglycosylation protocol is for use with New England Biolabs’ Rapid PNGase F. Learn more about Rapid PNGase F.