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Markers & Ladders

DNA Molecular Weight Markers

New England Biolabs sells a wide range of double-stranded DNA molecular weight markers for conventional electrophoresis. Conventional electrophoresis markers include: HindIII (NEB #N3012) and BstEII (NEB #N3014) digests of Lambda DNA, BstNI (NEB #N3031) and MspI (NEB #N3032) digests of pBR322 DNA, and a HaeIII (NEB #N3026) digest of φX174 DNA. These markers have a size range of approximately 10 base pairs to 23,000 base pairs. Our Lambda DNA–Mono Cut Mix (NEB #N3019) is designed to be used as an RFLP marker on Southern blots because it provides an uncomplicated gel pattern. 

We also supply a series of DNA ladders ranging from 25 bp to 48.5 kb. NEB’s DNA ladders are available in 4 formats: Conventional (NEB #N3231, NEB #N3232, NEB #N3200), Quick-Load® (NEB #N0467, NEB #N0468, NEB #N0469) with bromophenol blue tracking dye, Quick Load Purple (NEB #N0550, NEB #N0551, NEB #N0552, NEB #N0556, NEB #N3271, NEB #N3272, NEB #N3270) containing three dyes to track gel migration. NEB's DNA ladders feature exceptional quality and value. With our new Quick-Load Purple DNA Ladders (NEB #N0550, NEB #N0551, NEB #N0552), you'll appreciate the crisp bands and improved visibility, since there's no UV shadow.


RNA Markers and Ladders

Our RNA ladders and markers have a size range of up to 9,000 bases. These include the Low Range ssRNA Ladder (NEB #N0364), ssRNA Ladder (NEB #N0362), dsRNA Ladder (NEB #N0363), and microRNA Marker (NEB #N2102).


Protein Standards

New England Biolabs offers a selection of highly pure protein standards. Sizes range from 10 to 250 kDa which is ideal for accurate molecular weight determination for a wide range of expressed proteins. We offer a blue prestained protein standard, as well as a colored prestained protein standard with multi-colored bands for easy identification. Standards are provided pre-mixed with loading buffer and reducing agent.

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Markers & Ladders includes these subcategories:

DNA Markers & Ladders
Protein Standards
Quick-Load Purple DNA Ladders
RNA Markers & Ladders

FAQs for Markers & Ladders

Protocols for Markers & Ladders

    Other Tools & Resources


  • PCR Brochure

    The PCR brochure provides product information on the wide range of DNA polymerases available from NEB, including tools for selection and troubleshooting tips.

  • Protein Expression & Purification Brochure

    The Protein Expression and Purification brochure provides information on the advanced tools for protein expression and purification offered by NEB.

Comparison of NEB to Other Commercially Available DNA Ladders

Comparison of the NEB 1kb (left) and 100bp (right) DNA Ladders to other commercially available DNA Ladders. Note: The 500 bp DNA Ladder from Roche is used in the 1 kb DNA Ladder comparison.

DNA Markers & Ladders

DNA Markers & Ladders Selection Chart

NEB offers a variety of DNA ladders with sizes ranging from 25 bp to 40 kb for use in agarose gel electrophoresis. NEB also offers double-stranded DNA molecular weight markers for conventional with sizes ranging from 10 bp to 23,000 base pairs for use in agarose gel electrophoresis.

Protein Markers & Ladders

protein ladders

RNA Markers & Ladders