Markers & Ladders

DNA Molecular Weight Markers

New England Biolabs sells a wide range of double-stranded DNA molecular weight markers for conventional electrophoresis. Conventional electrophoresis markers include: HindIII (NEB #N3012) and BstEII (NEB #N3014) digests of Lambda DNA, BstNI (NEB #N3031) and MspI (NEB #N3032) digests of pBR322 DNA, and a HaeIII (NEB #N3026) digest of φX174 DNA. These markers have a size range of approximately 10 base pairs to 23,000 base pairs. Our Lambda DNA–Mono Cut Mix (NEB #N3019) is designed to be used as an RFLP marker on Southern blots because it provides an uncomplicated gel pattern. 

We also supply a series of DNA ladders ranging from 25 bp to 48.5 kb. NEB’s DNA ladders are available in 4 formats: Conventional (NEB #N3231, NEB #N3232, NEB #N3200), Quick-Load® (NEB #N0467, NEB #N0468, NEB #N0469) with bromophenol blue tracking dye, Quick Load Purple (NEB #N0550, NEB #N0551, NEB #N0552, NEB #N0556, NEB #N3271, NEB #N3272, NEB #N3270) containing three dyes to track gel migration. NEB's DNA ladders feature exceptional quality and value. With our new Quick-Load Purple DNA Ladders (NEB #N0550, NEB #N0551, NEB #N0552), you'll appreciate the crisp bands and improved visibility, since there's no UV shadow.

RNA Markers and Ladders

Our RNA ladders and markers have a size range of up to 9,000 bases. These include the Low Range ssRNA Ladder (NEB #N0364), ssRNA Ladder (NEB #N0362), dsRNA Ladder (NEB #N0363), and microRNA Marker (NEB #N2102).

Protein Standards

New England Biolabs offers a selection of highly pure protein standards. Sizes range from 10 to 250 kDa which is ideal for accurate molecular weight determination for a wide range of expressed proteins. We offer a blue prestained protein standard, as well as a colored prestained protein standard with multi-colored bands for easy identification. Standards are provided pre-mixed with loading buffer and reducing agent.