Nucleic Acid Purification
Biological workflows for detecting, analyzing, amplifying or manipulating DNA and RNA require extraction and purification from a biological sample and/or enzymatic reactions. DNA and RNA share similar biochemical structures, so purification methods are similar.
Monarch nucleic acid purification kits provide fast and reliable purification of high quality DNA and RNA from a variety of sources using best-in-class silica column technology. DNA and RNA purified with Monarch Kits is highly-pure and suitable for use in a wide variety of downstream applications including sequencing, cloning, and enzymatic manipulations. Available for plasmid minipreps, DNA gel extraction, PCR & DNA cleanup and total RNA extraction, Monarch kits are all designed with sustainability in mind; they use less plastic and are packaged in responsibly-sourced, recyclable material.
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Nucleic Acid Purification includes these subcategories:
FAQs for Nucleic Acid Purification
- What is the maximum binding capacity of the Monarch DNA Cleanup Column provided in the Monarch DNA Gel Extraction Kit?
- I have used 5 ml of cell culture for plasmid isolation with the Monarch Plasmid Miniprep kit and I am obtaining low amounts of plasmid and/or contaminating gDNA. Why is this, and what are your suggestions to improve yield and purity?
- Are plasmids recovered using the Monarch Plasmid Miniprep Kit endotoxin free?
- Can I use water to elute the DNA when using the Monarch Kits?
- What is the maximum binding capacity of the Monarch DNA Cleanup Column provided with the Monarch PCR & DNA Cleanup Kit (5 μg)?
- Are Monarch spin columns compatible with Vacuum Manifolds?
- Can I use the Monarch DNA & PCR Cleanup Kit to purify oligonucleotides and other short DNA fragments?
Protocols for Nucleic Acid Purification
- Monarch® Plasmid DNA Miniprep Kit Protocol (NEB #T1010)
- Quick Protocol for Monarch® Plasmid Miniprep Kit (NEB #T1010)
- Quick Protocol for Monarch® DNA Gel Extraction Kit (NEB #T1020)
- Monarch® DNA Gel Extraction Kit Protocol (NEB #T1020)
- Removal of Residual gDNA after Purification of Low Copy Plasmid using Exonuclease V (RecBCD)
- Quick Protocol for DNA Cleanup and Concentration Using the Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030)
- Protocol for DNA Cleanup and Concentration Using the Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030)
- Quick Protocol for Oligonucleotide Cleanup Using the Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030)
- Oligonucleotide Cleanup Using Monarch® PCR & DNA Cleanup Kit (5 μg) Protocol (NEB #T1030)
- Total RNA Purification from Mammalian Whole Blood Samples using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Tough-to-Lyse Samples (bacteria, yeast, plant, etc.) using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Samples Stored in Monarch DNA/RNA Protection Reagent using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Cultured Mammalian Cells using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Tissues and Leukocytes using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- In-tube DNase I Treatment after Purification with the Monarch Total RNA Miniprep Kit (NEB #T2010)
- RNA Reaction Cleanup using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Fractionation of Small and Large RNA Using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from Samples in RNAlater® using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Total RNA Purification from TRIzol®-extracted Samples using the Monarch Total RNA Miniprep Kit (NEB #T2010)
- Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010)
- Using Exonuclease V (RecBCD) to Eliminate Residual Genomic DNA When Purifying Low Copy Plasmids with the Monarch® Plasmid Miniprep Kit
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Monarch Nucleic Acid Purification Brochure
It’s time to transform your DNA and RNA purification experience! Learn about the advantages of choosing Monarch Nucleic Acid Purification Kits from NEB.
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- Troubleshooting Guide for DNA Cleanup and Plasmid Purification
- Troubleshooting Guide for RNA Cleanup
- Troubleshooting Guide for Total RNA Extraction & Purification
- Back to basics: Important things to keep in mind when purifying plasmids and DNA fragments
- Dos and Don'ts of Plasmid Minipreps
- Six Tips for a Perfect Gel Extraction
- Tips for the Monarch PCR & DNA Cleanup Kit
- Usage Guidelines for the Monarch Plasmid Miniprep Kit (#T1010) When Working with Low Copy Plasmids
Other Tools & Resources
Application Notes
Brochures
Feature Articles
Troubleshooting Guides
Usage Guidelines
Product Manual
Legal Information
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Videos
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Monarch Plasmid Miniprep Kit protocol
Learn how to do a plasmid miniprep using the Monarch Plasmid Miniprep Kit.
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Monarch DNA Gel Extraction Kit protocol
Learn how to extract DNA from agarose gels using the Monarch DNA Gel Extraction Kit.
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Monarch® PCR & DNA Cleanup Kit Protocol
Learn how to isolate DNA from your enzymatic reactions, including PCR, using the Monarch PCR & DNA Cleanup Kit (5 µg).
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Tips for using the Monarch Plasmid Miniprep Kit
Optimize your plasmid miniprep results with these quick tips for using the Monarch Plasmid Miniprep Kit.
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Tips for using the Monarch DNA Gel Extraction Kit
Optimize your DNA gel extractions with our quick tips for using the Monarch DNA Gel Extraction Kit.
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Tips for using the Monarch® PCR & DNA Cleanup Kit
Optimize your DNA isolation from PCR and other enzymatic reactions with our quick tips for using the Monarch PCR & DNA Cleanup Kit.
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Monarch Total RNA Miniprep Protocol
This video walks you through RNA purification from a wide variety of sample types using the Monarch Total RNA Miniprep Kit.
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Tips for Successful RNA Purification using Monarch Total RNA Miniprep Kit
Optimize the results of your RNA extractions with these simple tips from our scientists.
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How to recycle your Monarch Kit components
Learn how you can easily recycle all of the components in your Monarch Kits.
