NEBNextDirect_CustomReady_LP_Banner_0418

Employing the unique NEBNext Direct® hybridization-based enrichment method, NEBNext Direct Custom Ready Panels allow customization of targeted gene panels for Illumina® sequencing. Select from a list of genes for which baits have been carefully designed and optimized to give complete coverage of the full coding regions. High quality panels can be designed by you and rapidly delivered, from any combination of genes. NEBNext Direct Custom Ready Panels provide the content you want with the performance you need.

  1. NEBNext_Direct_Webinar_282x210

    Challenges and Opportunities for Next Generation Sequencing Target Enrichment

    Target enrichment offers several advantages over more comprehensive genomic profiling for a variety of scientific and applications. During this webinar, we explore the advantages and limitations of target enrichment, and discuss how NEBNext Direct®, a novel solution for selective enrichment of genomic targets, addresses these challenges.

  2. NEBNext Direct Workflow

    NEBNext Direct® Workflow

    View our tutorial for help visualizing NEBNext Direct’s unique and enabling workflow.

  3. NEB TV

    NEB TV Ep. 11 – Target Enrichment for NGS

    Precision medicine requires precision enrichment. Learn more about target enrichment and its role in clinical applications.

  4. NEBNext Direct Target Enrichment

    NEBNext Direct® Target Enrichment

    Learn about the innovative NEBNext Direct technology for target enrichment for next generation sequencing.

NEBNext Direct Workflow

Fig1_NEBNext_Direct_CR_Workflow

 


NEBNext Direct Custom Ready Panels demonstrate optimum performance across a wide range of panel sizes

Fig4_NEBNextDirectCR_Performance

Key target enrichment metrics demonstrate consistent performance across a range of panel sizes. 100 ng of DNA was tested against panels of 1, 10, 25, 50 and 100 genes, and sequenced using Illumina paired-end 150 bp sequencing. Larger panels included all genes present in smaller panels.


NEBNext Direct Custom Ready Panels demonstrate retention of target behavior across panel sizes.

Fig3_NEBNextDirectCR_IGV

IGV image of coverage profile for 4 BRAF exons included in panels of 1, 10, 25, 50 and 100 genes, demonstrate consistent target behavior with the addition of gene targets. 100 ng of DNA was used as input for NEBNext enrichment using the 5 panels, including the BRAF gene. Libraries were sequenced on an Illumina 2 x 150 basepair sequencing.


NEBNext Direct Nicking Enzyme produces consistent fragmentation across DNA input quantities.

Fig5_NEBNextDirectCR_NickingOverlay

10 ng, 100 ng, and 1 µg of Control DNA was used as input for fragmentation using the NEBNext Direct Nicking Enzyme. Representative electropherograms show fragment sizes compatible with NEBNext Direct enrichment across DNA input amounts.


Use our NEBNext Direct®Custom Ready Panel Request Form to order.

Click Here

Try NEBNext Direct®
for yourself!

Request a sample

Advantages

  • Choose from a single gene to hundreds of genes

  • Experience unmatched specificity and coverage uniformity

  • Eliminate synthesis and optimization steps for faster turnaround

  • Improve sensitivity with our Unique Molecule Index (UMI)

  • Generate results in one day with our automation-friendly workflow  

NEBNext Direct Custom Ready Panels have allowed my research to focus on the specific genes we need to explore. In addition to the convenience of easily selecting genes for focused panels, NEBNext Direct enrichment has provided the necessary reliability and depth of coverage to enable robust somatic variant calling."

– Guang Peng, M.D., PhD,
   MD Anderson Cancer    Center

What People are Saying

NEBNext Direct enrichment technology is by far the fastest and most automation friendly protocol available today. I can have samples on the sequencer in 6 hours starting from genomic DNA. The technology produces very high on target percentages (>90%) for even very small panels, and in combination with molecular barcoding produces low duplication rates. From an optimization perspective, NEBNext Direct enrichment allows me to assign individual captured fragments to a probe unambiguously, thus giving the opportunity for optimizing the coverage distribution of any target.

– Eric C. Olivares,
   Founder, SEQanswers.com

NEB was fantastic while developing our panel or updating an existing one. The protocol is simple and fast and the results have been superb.

– Luca Magnani, Ph.D
   CRUK Fellow
   Department of Surgery and    Cancer
   Imperial Centre for    Translational and    Experimental Medicine

NEW ENGLAND BIOLABS® and NEB® are registered trademarks of New England Biolabs, Inc.
NEBNext Direct® is a trademark of New England Biolabs, Inc.
Directed Genomics™ is a trademark of Directed Genomics