RNA Polymerase Selection Chart

T7 and SP6 RNA polymerases are DNA dependent RNA polymerases that produce DNA templated RNA transcripts. T7 and SP6 exhibit high specificity for their respective promoters. Both T7 and SP6 can be used for the in vitro synthesis of RNA for a wide variety of applications, including transfection, translation, structural studies and radioactive and non-isotopic probe generation. E. coli Poly(A) Polymerase and Poly(U) Polymerase generate untemplated homoribopolymeric tails on RNA 3´-ends. Both E. coli Poly(A) Polymerase and Poly(U) Polymerase can be used for RNA tailing prior to reverse transcription or for direct labeling.

Product NEB # Applications Size
T7 RNA Polymerase M0251S
M0251L
  • Radiolabeled RNA probe preparation
  • RNA generation for in vitro translation
  • RNA generation for studies of RNA structure, processing and catalysis
  • Expression control via anti-sense RNA
5,000 units
25,000 units
SP6 RNA Polymerase M0207S
M0207L
  • Radiolabeled RNA probe preparation (2)
  • RNA generation for in vitro translation (3)
  • RNA generation for studies of RNA structure, processing and catalysis (3)
  • Expression control via anti-sense RNA
2,000 units
10,000 units
E. coli RNA Polymerase, Core Enzyme M0550S
  • RNA synthesis from E. coli promoter
  • Transcription initiation studies
  • In vitro translation with PURExpress
100 units
E. coli RNA Polymerase, Holoenzyme M0551S
  • RNA synthesis from E. coli promoter 
  • Transcription initiation studies
  • In vitro translation with PURExpress
50 units
E. coli Poly(A) Polymerase M0276S
M0276L
  • Labeling of RNA with ATP or cordycepin
  • Poly(A) tailing of RNA for cloning or affinity purification
  • Enhances translation of RNA transferred into eukaryotic cells.
100 units
500 units
Poly(U) Polymerase M0337S
  • Labeling of RNA with UTP
  • Poly(U) and Poly(A) tailing of RNA for cloning
  • Studying effects of poly(U) tailing on stability and translation of RNA transferred into eukaryotic cells
  • Poly (A) tailing of 2' O-Me modified 3' ends
60 units