New England Biolabs Canada
 

Product Pathways - Protein Folding

HSP90 Antibody #4874

Item# Description List Price Web Price Qty
4874S HSP90 Antibody - 100 µl $383.00
$344.70
ADD TO CART
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
X

Companion Products

Item# Description List Price Web Price Qty
12630P SignalFire™ Plus ECL Reagent - 20 ml $120.00
$108.00
ADD TO CART
12630S SignalFire™ Plus ECL Reagent - 100 ml $519.00
$467.10
ADD TO CART
12757P SignalFire™ Elite ECL Reagent - 20 ml $127.00
$114.30
ADD TO CART
12757S SignalFire™ Elite ECL Reagent - 100 ml $558.00
$502.20
ADD TO CART
14746P SignalStain® Citrate Unmasking Solution (10X) - 25 ml $37.00
$33.30
ADD TO CART
14746S SignalStain® Citrate Unmasking Solution (10X) - 125 ml $137.00
$123.30
ADD TO CART
15019L Animal-Free Blocking Solution (5X) - 250 ml $199.00
$179.10
ADD TO CART
15019S Animal-Free Blocking Solution (5X) - 25 ml $59.00
$53.10
ADD TO CART
2401L Phospho-HSP27 (Ser82) Antibody - 300 µl $1,057.00
$951.30
ADD TO CART
2401S Phospho-HSP27 (Ser82) Antibody - 100 µl $446.00
$401.40
ADD TO CART
2402S HSP27 (G31) Mouse mAb - 100 µl $383.00
$344.70
ADD TO CART
2402T HSP27 (G31) Mouse mAb - 20 µl $163.00
$146.70
ADD TO CART
2404S Phospho-HSP27 (Ser15) Antibody - 100 µl $446.00
$401.40
ADD TO CART
2404T Phospho-HSP27 (Ser15) Antibody - 20 µl $180.00
$162.00
ADD TO CART
2405S Phospho-HSP27 (Ser78) Antibody - 100 µl $446.00
$401.40
ADD TO CART
2405T Phospho-HSP27 (Ser78) Antibody - 20 µl $180.00
$162.00
ADD TO CART
4872S HSP70 Antibody - 100 µl $383.00
$344.70
ADD TO CART
4872T HSP70 Antibody - 20 µl $163.00
$146.70
ADD TO CART
4875S HSP90 (E289) Antibody - 100 µl $383.00
$344.70
ADD TO CART
4877S HSP90 (C45G5) Rabbit mAb - 100 µl $383.00
$344.70
ADD TO CART
4877T HSP90 (C45G5) Rabbit mAb - 20 µl $163.00
$146.70
ADD TO CART
5425S Normal Goat Serum - 10 ml $94.00
$84.60
ADD TO CART
6883P3 SignalFire™ ECL Reagent - 50 ml $44.00
$39.60
ADD TO CART
6883S SignalFire™ ECL Reagent - 500 ml $382.00
$343.80
ADD TO CART
7074P2 Anti-rabbit IgG, HRP-linked Antibody - 100 µl $112.00
$100.80
ADD TO CART
7074S Anti-rabbit IgG, HRP-linked Antibody - 1 ml $209.00
$188.10
ADD TO CART
7074V Anti-rabbit IgG, HRP-linked Antibody - 5 ml $672.00
$604.80
ADD TO CART
7727L Biotinylated Protein Ladder Detection Pack - 3250 µl $336.00
$302.40
ADD TO CART
7727S Biotinylated Protein Ladder Detection Pack - 650 µl $94.00
$84.60
ADD TO CART
8059P SignalStain® DAB Substrate Kit - 1 Kit $61.00
$54.90
ADD TO CART
8059S SignalStain® DAB Substrate Kit - 1 Kit $206.00
$185.40
ADD TO CART
9997S Tris Buffered Saline with Tween® 20 (TBST-10X) - 1000 ml $93.00
$83.70
ADD TO CART
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Source
W Human, Mouse, Rat, Monkey, D. melanogaster, Zebrafish Endogenous 90 Rabbit
IHC-P

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin)

Homology

Species predicted to react based on 100% sequence homology: Chicken, Bovine.

Specificity / Sensitivity

HSP90 Antibody detects endogenous levels of total HSP90 protein, alpha and beta isoforms. This antibody does not cross-react with other HSPs.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to human HSP90. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells using HSP90 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HSP90 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic and nuclear localization, using HSP90 Antibody.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using HSP90 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using HSP90 Antibody.

Background

HSP70 and HSP90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress (1). Both HSP70 and HSP90 are able to interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of their substrates in an ATP- and co-chaperone-dependent manner (1). HSP70 has a broad range of substrates including newly synthesized and denatured proteins, while HSP90 tends to have a more limited subset of substrates, most of which are signaling molecules. HSP70 and HSP90 often function collaboratively in a multi-chaperone system, which requires a minimal set of co-chaperones: HSP40, Hop, and p23 (2,3). The co-chaperones either regulate the intrinsic ATPase activity of the chaperones or recruit chaperones to specific substrates or subcellular compartments (1,4). When the ubiquitin ligase CHIP associates with the HSP70/HSP90 complex as a cofactor, the unfolded substrates are subjected to degradation by the proteasome (4). The biological functions of HSP70/HSP90 extend beyond their chaperone activity. They are essential for the maturation and inactivation of nuclear hormones and other signaling molecules (1,3). They also play a role in vesicle formation and protein trafficking (2).

Application References

  • Jo, S.K. et al. (2006) J Am Soc Nephrol 17, 3082-92. Applications: Western Blotting.
  • Izumi, N. et al. (2012) Cancer Sci 103, 50-7. Applications: Western Blotting.
  • Rao, T. et al. (2014) Breast Cancer Res 16, R11. Applications: Western Blotting.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

Toll Free: 1-800-387-1095

Fax: 1-800-563-3789

info.ca@neb.com

orders.ca@neb.com

About NEBTerms of Sale Web Discount Shipping Information Web Site Disclaimer Privacy Policy NEB USA Cell Signaling Technology Sitemap
Contents ¬©New England Biolabs Ltd.  New England Biolabs Ltd. is the exclusive Canadian distributor for Cell Signaling Technology, Inc.  New England Biolabs, Inc. is an ISO 9001 certified company.

Search another product:

 

Item has been added to the cart

 

Item has been added to the favourites