NEB New Products

NEBNext Ultrashear™ FFPE DNA Library Prep Kit

This kit for FFPE DNA library prep combines specialized enzymatic FFPE DNA fragmentation and repair, with optimized reagents and protocols to provide superior performance with this challenging sample type.

NEBNext^® FFPE DNA Library Prep Kit

This kit is designed for NGS library prep of pre-sheared FFPE DNA and includes reagents for repair and optimized library prep.

NEBNext UltraShear™

This new enzyme mix for DNA fragmentation has been designed for use with challenging samples, including FFPE DNA and methylated DNA. NEBNext UltraShear maintains methylation marks on DNA.

EnGen^® Seq1 Cas9

EnGen Seq1 Cas9 from Streptococcus equinus is an RNA-guided DNA endonuclease that catalyzes site-specific cleavage of double-stranded DNA (dsDNA).

NEBNext^® Multiplex Oligos for Illumina^® (Unique Dual Index UMI Adaptors DNA Set 2)

The second set of Unique Dual Index UMI Adaptors for improved SNV detection or PCR-free workflows.

NEBNext^® Multiplex Oligos for Illumina^® (Unique Dual Index UMI Adaptors DNA Set 3)

The third set of Unique Dual Index UMI Adaptors for improved SNV detection or PCR-free workflows.

NEBNext^® Multiplex Oligos for Illumina^® (Unique Dual Index UMI Adaptors DNA Set 4)

The fourth set of Unique Dual Index UMI Adaptors for improved SNV detectiPCRon or PCR-free workflows.

NEBExpress^® T4 Lysozyme

NEBExpress^® T4 Lysozyme is a recombinant murein hydrolase that breaks down the bacterial cell wall by hydrolyzing the -1,4 linkage between N-acetylmuramic acid and N-acetylglucosamine in the peptidoglycan of prokaryotic cells (gram-negative and some gram-positive bacteria).  It can be used to extract soluble proteins, membrane proteins, DNA, RNA, or metabolites.

phi29-XT RCA Kit

This kit contains the components for optimized RCA: phi29-XT DNA Polymerase engineered for improved performance, exo-resistant random primers, and dNTPs.

NEBNext^® High Input Poly(A) mRNA Isolation Module

Isolate intact poly(A)+ RNA from high inputs (5-50 µg) of previously isolated total RNA, using oligo d(T)25-coupled paramagnetic beads. Intact mRNA can be obtained in approximately one hour, and eluted in small volumes. 

Thermostable OGG

Thermostable OGG is a bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities.

Thermostable USER^® III Enzyme

Thermostable USER III Enzyme generates a single nucleotide gap at the location of a uracil residue. It is active between 50-75°C.

Ph.D.™-C7C Phage Display Peptide Library Kit v2

The Ph.D.-C7C Phage Display Peptide Library is based on a combinatorial library of random disulfide looped peptides fused to the N-terminus of a minor coat protein (pIII) of M13 phage. The Ph.D.-C7C™ Kit v2 contains the Ph.D.-C7C Phage Display Peptide Library, a DYKDDDDK Mouse monoclonal antibody and Protein G Magnetic Beads for a panning control experiment, and enough -96gIII sequencing primer for >50 sequencing reactions.

Induro^® Reverse Transcriptase

Msz Exonuclease I

Msz exonuclease I is a DNA-specific exonuclease that removes nucleotides from linear single-stranded DNA in the 3′ to 5′ direction.

Ph.D™.-12 Phage Display Peptide Library Kit v2

The Ph.D.-12 Phage Display Peptide Library is a combinatorial library of random 12-mer peptides fused to the N-terminus of a minor coat protein (pIII) of M13 phage. The Ph.D.-12™ Kit v2 contains the Ph.D.-12 Phage Display Peptide Library, a DYKDDDDK Mouse monoclonal antibody and Protein G Magnetic Beads for a panning control experiment, and enough -96gIII sequencing primer for >50 sequencing reactions.

Ph.D.™-7 Phage Display Peptide Library Kit v2

The Ph.D.-7 Phage Display Peptide Library is a combinatorial library of random 7-mer peptides fused to the N-terminus of a minor coat protein (pIII) of M13 phage. The Ph.D.-7™ Kit v2 contains the Ph.D.-7 Phage Display Peptide Library, a DYKDDDDK Mouse monoclonal antibody and Protein G Magnetic Beads for a panning control experiment, and enough -96gIII sequencing primer for >50 sequencing reactions.

DNase I-XT

DNase I-XT is a salt-tolerant DNA endonuclease that nonspecifically cleaves DNA, and is ideal for in vitro transcription and RNA preps.

Faustovirus Capping Enzyme

FCE adds the m⁷G-cap to the 5′ end of triphosphorylated and dephosphorylated RNA. This enzyme offers improved capping efficiency under a wide range of temperatures, and is ideal for capping prior to translation assays/in vitro translation.

Q5^® Blood Direct 2X Master Mix

Q5^® Blood Direct 2X Master Mix can amplify a wide variety of targets direct from dried blood spots or up to 30% whole human blood, allowing you to skip DNA purification.

WarmStart^® Nt.BstNBI

This nicking endonuclease recognizes the site GAGTC(4/-5) and is inhibited at temperatures below 40°C, enabling room temperature setup.

LunaScript^® RT SuperMix

 LunaScript RT SuperMix is an optimized master mix containing all the necessary components for first strand cDNA synthesis, except RNA template. Supermix only version.

NEBNext^® FFPE DNA Repair v2 Module

This product enables efficient and streamlined repair of FFPE DNA in next generation sequencing workflows.

NEBNext^® Multiplex Oligos for Illumina^® (96 Unique Dual Index Primer Pairs Set 5)

The fifth set of unique dual index primer pairs in the NEBNext portfolio, enabling high-efficiency adaptor ligation, minimized adaptor-dimer formation, and filtered index-hopped reads.

LyoPrime Luna^® Probe One-Step RT-qPCR Mix with UDG

LyoPrime Luna™ One-Step RT-qPCR Master Mix with UDG enables sensitive detection of target RNA sequences and is supplied in a lyophilized format for room temperature shipping and storage.

NEBNext^® Library Quant DNA Standards

This product contains additional volume of the six library quantification standards included with the NEBNext Library Quant Kit for Illumina.

Luna^® Probe One-Step RT-qPCR 4X Mix with UDG (No ROX)

This 4X RT-qPCR mix enables higher amounts of sample input, making it ideal for molecular diagnostic applications. This mix does not contain a passive reference dye.

NEBNext^® Ultra™ II FS DNA PCR-free Library Prep with Sample Purification Beads

The NEBNext Ultra II FS DNA PCR-free Library Prep Kit utilizes an amplification-free workflow for enzymatic fragmentation and DNA-seq, based on NEBNext Ultra II technology. The kit is provided with SPRIselect sample cleanup beads.

NEBNext^® Ultra™ II FS DNA PCR-free Library Prep Kit for Illumina^®

The NEBNext Ultra II FS DNA PCR-free Library Prep Kit for Illumina utilizes an amplification-free workflow for enzymatic fragmentation and DNA-seq, based on NEBNext Ultra II technology. This kit is provided without cleanup beads.

NEBNext^® Ultra™ II DNA PCR-free Library Prep with Sample Purification Beads

NEBNext Ultra II DNA PCR-free Library Prep with Sample Purification Beads utilizes an amplification-free workflow for DNA-seq, based on NEBNext Ultra II technology. This kit is provided with SPRIselect sample cleanup beads.

NEBNext^® Ultra™ II DNA PCR-free Library Prep Kit for Illumina^®

The NEBNext Ultra II DNA PCR-free Library Prep Kit utilizes an amplification-free workflow for DNA-seq, based on NEBNext Ultra II technology. This kit is provided without cleanup beads.

HiScribe^® T7 mRNA Kit with CleanCap^® Reagent AG

The HiScribe^®; T7 mRNA Kit with Clean Cap^® Reagent AG is designed for the high yield in vitro transcription of capped mRNA using T7 RNA Polymerase.

NEBExpress^® E. coli Lysis Reagent

NEBExpress^® E. coli Lysis Reagent is a chemical lysis solution composed of a proprietary mix of non-ionic and zwitterionic detergents and Tris-based buffer. It allows disruption of E. coli cells without denaturing soluble proteins. 

NEBridge^® Ligase Master Mix

Use NEBridge Ligase Master Mix with your choice of NEB Type IIS restriction enzyme for a convenient, efficient and accurate Golden Gate Assembly.

LunaScript^® Multiplex One-Step RT-PCR Kit

This kit enables a streamlined one step RT-PCR protocol using Luna WarmStart Reverse Transcriptase and Q5 Hot Start High-Fidelity DNA Polymerase.