HpaII Methyltransferase

Catalog # Concentration Size List Price Quantity Your Price
M0214S 4000 units/ml 100 units $116.00
$104.40
Catalog # Size List Price Your Price
M0214S 100 units $116.00
$104.40
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

HpaII Methyltransferase recognizes the same sequence as the MspI Methyltransferase, but modifies the internal cytosine residue (C5) of the sequence CCGG.

Product Source
An E. coli strain that carries the cloned HpaII modification gene from Haemophilus parainfluenzae (ATCC 49669).
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  HpaII Methyltransferase M0214SVIAL -20 1 x 0.025 ml 4,000 units/ml
  rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X
  S-adenosylmethionine (SAM) B9003SVIAL -20 1 x 0.1 ml 32 mM

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to protect 1 μg λ DNA in 1 hour at 37°C in a total reaction volume of 10 μl against cleavage by HpaII restriction endonuclease.

Reaction Conditions

1X rCutSmart™ Buffer
Supplement with 80 µM S-adenosylmethionine (SAM)
Incubate at 37°C

1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Control Protection Assay

HpaII Methyltransferase is incubated with 1 µg of λ DNA in 10 µl 1X rCutSmart Buffer, supplemented with 80 µM S-adenosylmethionine, for one hour at 37°C followed by 15 minutes at 65°C. The extent of protection by HpaII Methyltransferase is determined by the addition of 40 µl NEBuffer 1 supplemented with 10 mM MgCl2 and 10 units of HpaII restriction endonuclease. Incubation at 37°C for 30 minutes is followed by analysis on agarose gels.

Storage Buffer

50 mM Tris-HCl
150 mM NaCl
0.1 mM EDTA
5 mM TCEP
200 µg/ml BSA
50% Glycerol
pH 7.5 @ 25°C

Heat Inactivation

65°C for 20 minutes

Molecular Weight

Theoretical: 40397 daltons


Materials Sold Separately
References
  • Hoffman, J.L. (1986). Biochemistry. 25, 4444-4449.
Quality Control Assay
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Specifications
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Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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