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T7 Exonuclease

Catalog # Concentration Size List Price Quantity Your Price
M0263L 10000 units/ml 5000 units $431.00
$387.90
M0263S 10000 units/ml 1000 units $107.00
$96.30
Catalog # Size List Price Your Price
M0263L 5000 units $431.00
$387.90
M0263S 1000 units $107.00
$96.30
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

  • Double-stranded DNA specific exonuclease
  • Initiates at the 5' termini of linear or nicked double-stranded DNA
  • Catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in the 5' to 3' direction
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

T7 Exonuclease is ideal for:

  • Site-directed mutagenesis
  • Nick-site extension 
Featured Videos
View Video Library
  • Monarch_MiniprepProtocol_VideoThumb
    What are exonucleases and their applications?
  • Monarch_MiniprepProtocol_VideoThumb
    Overview of the Q5® Site-Directed Mutagenesis Kit
  • Monarch_MiniprepProtocol_VideoThumb
    Troubleshooting tips for Q5® Site-Directed Mutagenesis Kit
Highlights
  • Isolated from a recombinant source
  • 5' -> 3' exonuclease
  • Supplied with 10X Reaction Buffer
Product Source
Purified from an E. coli strain containing a TYB12 intein fusion.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  T7 Exonuclease M0263SVIAL -20 1 x 0.1 ml 10,000 units/ml
  NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X
  T7 Exonuclease M0263LVIAL -20 1 x 0.5 ml 10,000 units/ml
  NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble deoxyribonucleotide in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X NEBuffer 4 with 0.15 mM sonicated duplex [3H]-DNA.

Reaction Conditions

1X NEBuffer™ 4
Incubate at 25°C

1X NEBuffer™ 4
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
1 mM DTT
(pH 7.9 @ 25°C)

Activity in NEBuffers

NEBuffer™ 1: 30%
NEBuffer™ 2: 25%
NEBuffer™ 3: 1%
NEBuffer™ 4: 100%

Storage Buffer

10 mM Tris-HCl
5 mM DTT
0.1 mM EDTA
50% Glycerol
pH 8 @ 25°C

Heat Inactivation

No

Companion Products
Materials Sold Separately
References
  • Kerr, C. and Sadowski, P.D. (1972). J. Biol. Chem. 247, 305-318.
  • Shinozaki, K. and Okazaki, T. (1978). Nucl. Acids. Res. 5, 4245-4261.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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