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Msz Exonuclease I

Catalog # Concentration Size List Price Quantity Your Price
M0527S 10000 units/ml 1000 units $113.00
$101.70
Catalog # Size List Price Your Price
M0527S 1000 units $113.00
$101.70
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

M0527 Mechanism

  • DNA-specific exonuclease
  • Can be heat inactivated at 80°C in 1 minute
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

Msz Exonuclease I (MszExoI) is Ideal for: 

  • Removal of linear single-stranded DNA oligos in thermophilic workflow applications within 45°C and 60°C.

  • This is an Enzyme for Innovation (EFI). EFI is a project initiated by NEB to provide unique enzymes to the scientific community in the hopes of enabling the discovery of new and innovative applications. These enzymes have interesting properties and unique specificities.
 
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Msz Exonuclease I is isolated from a strain of E. coli, which carries the cloned pMC45-1 gene from Methylocaldum szegediense. Msz Exonuclease I is a DNA specific exonuclease that catalyzes the removal of nucleotides from linear single-stranded DNA in the 3′ to 5′ direction with optimal activity between 45°C and 60°C in rCutSmart™ Buffer. 
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Msz Exonuclease I M0527SVIAL -20 1 x 0.1 ml 10,000 units/ml
  rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Companion Products
References
  • Fei L, Tian S, Moysey R, Misca M, Barker JJ, Smith MA, et al. (2015). Structural and Biochemical Studies of a Moderately Thermophilic Exonuclease I from Methylocaldum szegediense. PLoS ONE. 10 (2), e0117470. DOI: 10.1371/journal.pone.0117470
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Change Notifications
Effective 9-5-23, unit definition changed.
 
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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