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Product Pathways - Protein Stability

USP8 (D18F6) Rabbit mAb #11832

Item# Description List Price Web Price Qty
11832S USP8 (D18F6) Rabbit mAb - 100 µl $372.00
$334.80
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Monkey Endogenous 140 Rabbit IgG
IP

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation

Specificity / Sensitivity

USP8 (D18F6) Rabbit mAb recognizes endogenous levels of total USP8 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu614 of human USP8 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using USP8 (D18F6) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human USP8 (hUSP8-Myc/DDK; +), using USP8 (D18F6) Rabbit mAb.

Background

Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. The deubiquitinating enzyme ubiquitin-specific protease 8 (USP8/UBPy) is a cysteine protease belonging to the USP/UBP subfamily. Research studies have shown that USP8 is an essential growth-regulated enzyme indespensible for cell proliferation and survival (3,4). Indeed, conditional knock-out of murine USP8 was shown to promote a dramatic loss in expression of receptor tyrosine kinases, including EGFR, ErbB3, and c-Met (4). In agreement with these findings, USP8 inactivation leads to enhanced ubiquitination of ligand-activated EGFR (5,6). Furthermore, phosphorylation of USP8 at Ser680 results in its binding of 14-3-3, catalytic inactivation, and reduced EGFR deubiquitination (7). It appears as though USP8, in conjunction with components of the ESCRT-0 complex, plays an integral role in the early endosomal sorting machinery that functions to protect EGFR from lysosomal degradation (8,9).

  1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
  2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
  3. Naviglio, S. et al. (1998) EMBO J 17, 3241-50.
  4. Niendorf, S. et al. (2007) Mol Cell Biol 27, 5029-39.
  5. Alwan, H.A. and van Leeuwen, J.E. (2007) J Biol Chem 282, 1658-69.
  6. Mizuno, E. et al. (2005) Mol Biol Cell 16, 5163-74.
  7. Mizuno, E. et al. (2007) Exp Cell Res 313, 3624-34.
  8. Row, P.E. et al. (2006) J Biol Chem 281, 12618-24.
  9. Berlin, I. et al. (2010) J Biol Chem 285, 34909-21.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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