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Product Pathways - Protein Stability

β-TrCP (D12C8) Rabbit mAb #11984

Item# Description List Price Web Price Qty
11984S β-TrCP (D12C8) Rabbit mAb - 100 µl $372.00
$334.80
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse, Rat, Monkey Endogenous 62 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting

Protocols

Specificity / Sensitivity

β-TrCP (D12C8) Rabbit mAb recognizes endogenous levels of total β-TrCP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to human β-TrCP protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using β-TrCP (D12C8) Rabbit mAb.

Background

β-transducin repeat-containing protein (β-TrCP or FBW1A) is an F-box family protein characterized by the presence of the protein-protein mediating F-box domain first described in cyclin F. F-box proteins act as substrate adaptors that target proteins containing a specific phosphorylated sequence element, referred to as a phosphodegron, to the SCF E3 ubiquitin ligase complex for ubiquitination (1,2). β-TrCP targets many important proteins with diverse functions, such as p53, H-Ras, Smad4, IκBα, β-catenin, and the cell cycle checkpoint protein claspin, for ubiquitin-mediated degradation (3-5). Research studies have shown that inhibition of β-TrCP expression has a demonstrated benefit in the treatment of prostate cancer (6).

  1. Bour, S. et al. (2001) J Biol Chem 276, 15920-8.
  2. Kusmierczyk, A.R. and Hochstrasser, M. (2008) Biol Chem 389, 1143-51.
  3. Wan, M. et al. (2004) J Biol Chem 279, 14484-7.
  4. Mailand, N. et al. (2006) Mol Cell 23, 307-18.
  5. Frescas, D. and Pagano, M. (2008) Nat Rev Cancer 8, 438-49.
  6. Gluschnaider, U. et al. (2010) PLoS One 5, e9060.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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