New England Biolabs Canada
 

Product Pathways - Protein Folding

OS-9 (D8P4G) Rabbit mAb #12497

Item# Description List Price Web Price Qty
12497S OS-9 (D8P4G) Rabbit mAb - 100 µl $372.00
$334.80
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse, Rat, Monkey Endogenous 83,97 Rabbit IgG
IP

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation

Homology

Species predicted to react based on 100% sequence homology: Bovine, Horse.

Specificity / Sensitivity

OS-9 (D8P4G) Rabbit mAb recognizes endogenous levels of total OS-9 protein. This antibody reacts with OS-9 isoform 1 (OS-9-1) and OS-9 isoform 2 (OS-9-2).

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human OS-9 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using OS-9 (D8P4G) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or thapsigargin-treated (2 nM, 16 hr; +), using OS-9 (D8P4G) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

IP

IP

Immunoprecipitation of OS-9 from SJSA-1 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or OS-9 (D8P4G) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed with OS-9 (D8P4G) Rabbit mAb.


Background

Amplified in Osteosarcoma 9 (OS-9) was identified as a gene that is frequently amplified in human sarcomas (1). OS-9 is an N-glycosylated ER resident protein that harbors a mannose-6-phosphate receptor homology (MRH) domain and plays a critical role in mammalian ER quality control, in part, by functioning as a lectin. There are two major isoforms of OS-9 (OS-9-1, OS-9-2) that are generated through alternative splicing of mRNA (2). The expression of both OS-9 isoforms is enhanced upon activation of the IRE1/XBP1 pathway in cells exposed to acute ER stress. It is postulated that OS-9 serves as a retention factor for glycosylated and nonglycosylated, misfolded proteins, preventing their progression through the secretory pathway (3,4). Furthermore, OS-9 promotes the removal of misfolded proteins from the ER lumen and their subsequent proteasome-dependent degradation via its association with the Hrd1-SEL1L ubiquitin ligase complex (5).

  1. Su, Y.A. et al. (1996) Mol Carcinog 15, 270-5.
  2. Kimura, Y. et al. (1998) J Biochem 123, 876-82.
  3. Bernasconi, R. et al. (2008) J Biol Chem 283, 16446-54.
  4. Alcock, F. and Swanton, E. (2009) J Mol Biol 385, 1032-42.
  5. Christianson, J.C. et al. (2008) Nat Cell Biol 10, 272-82.

Application References

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