New England Biolabs Canada
 

Product Pathways - Innate Immunity

Mannose Receptor Antibody #12981

Item# Description List Price Web Price Qty
12981S Mannose Receptor Antibody - 100 µl $372.00
$334.80
ADD TO CART
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Source
W Human Endogenous 190 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting

Protocols

Specificity / Sensitivity

Mannose Receptor Antibody recognizes endogenous levels of total mannose receptor protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu115 of human mannose receptor protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Mannose Receptor Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

The mannose receptor (MR/CLEC13D/CD206/MMR/MRC1/Macrophage mannose receptor 1) is an endocytic receptor expressed by populations of dendritic cells, macrophages and nonvascular endothelium (1). The mannose receptor is a heavily glycosylated type I transmembrane protein with three types of extracellular domains and a short carboxy-terminal cytoplasmic domain with no apparent signaling motif (2-4). The extracellular portion of the protein is made up of a CR domain, which binds sulfated glycans, an FNII domain, which binds collagens, and eight C-type lectin domains, which bind carbohydrates containing mannose, fucose or GlcNAc (4-7). The receptor recycles between the plasma membrane and early endosomes (8). Functions include a role in antigen cross-presentation, clearance of endogenous proteins, pathogen detection and trafficking through lymphatic vessels (9-12).

  1. Martinez-Pomares, L. (2012) J Leukoc Biol 92, 1177-86.
  2. Lennartz, M.R. et al. (1989) J Biol Chem 264, 2385-90.
  3. Wileman, T.E. et al. (1986) Proc Natl Acad Sci U S A 83, 2501-5.
  4. Taylor, M.E. et al. (1990) J Biol Chem 265, 12156-62.
  5. Fiete, D.J. et al. (1998) Proc Natl Acad Sci U S A 95, 2089-93.
  6. Napper, C.E. et al. (2006) Biochem J 395, 579-86.
  7. Fiete, D. et al. (1997) Proc Natl Acad Sci U S A 94, 11256-61.
  8. Tietze, C. et al. (1982) J Cell Biol 92, 417-24.
  9. Burgdorf, S. et al. (2006) J Immunol 176, 6770-6.
  10. Lee, S.J. et al. (2002) Science 295, 1898-901.
  11. Milone, M.C. and Fitzgerald-Bocarsly, P. (1998) J Immunol 161, 2391-9.
  12. Marttila-Ichihara, F. et al. (2008) Blood 112, 64-72.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

Toll Free: 1-800-387-1095

Fax: 1-800-563-3789

info.ca@neb.com

orders.ca@neb.com

About NEBTerms of Sale Web Discount Shipping Information Web Site Disclaimer Privacy Policy NEB USA Cell Signaling Technology Sitemap
Contents ¬©New England Biolabs Ltd.  New England Biolabs Ltd. is the exclusive Canadian distributor for Cell Signaling Technology, Inc.  New England Biolabs, Inc. is an ISO 9001 certified company.

Search another product:

 

Item has been added to the cart

 

Item has been added to the favourites