New England Biolabs Canada
 
XP Monoclonal Antibody

Product Pathways - Lymphocyte Signaling

PD-L1 (E1L3N®) XP® Rabbit mAb #13684

Item# Description List Price Web Price Qty
13684S PD-L1 (E1L3N®) XP® Rabbit mAb - 100 µl $609.00
$548.10
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13684T PD-L1 (E1L3N®) XP® Rabbit mAb - 20 µl $207.00
$186.30
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human Endogenous 40-50 Rabbit IgG
IP
IHC-Bond
IHC-P
F

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-Bond=IHC-Leica® Bond™, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry

Specificity / Sensitivity

PD-L1 (E1L3N®) XP® Rabbit mAb recognizes endogenous levels of total PD-L1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PD-L1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from KARPAS-299, SUP-M2, and PC-3 cells using PD-L1 (E1L3N®) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from A549 cells, IFN-γ treated (100 ng/mL, 48 hr; +) or untreated (-), using PD-L1 (E1L3N®) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

IHC-Leica® Bond™

IHC-Leica® Bond™

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using PD-L1 (E1L3N®) XP® Rabbit mAb performed on the Leica® BOND™ Rx.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human placenta using PD-L1 (E1L3N®) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PD-L1 (E1L3N®) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Karpas-299 (left) or PC-3 (right) cell pellets using PD-L1 (E1L3N®) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Multiplex immunohistochemical analysis of paraffin-embedded human breast carcinoma usng PD-L1 (E1L3N®) XP® rabbit mAb (red), PD-1 (D4W2J) XP® rabbit mAb #86163 (green), LAG3 (D2G4O™) XP® rabbit mAb #15372 (magenta), TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow), CD8α (C8/144B) mouse mAb #70306 (orange), and Pan-keratin (C11) mouse mAb #4545 (cyan).

IHC-P (paraffin)

IHC-P (paraffin)

Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using PD-L1 (E1L3N®) XP® rabbit mAb (red), PD-L2 (D7U8C™) XP® rabbit mAb (magenta) #82723, Arginase-1 (D4E3M™) XP® rabbit mAb #93668 (green), IDO (D5J4E™) rabbit mAb #86630 (yellow), CD68 (D4B9C) XP® rabbit mAb #76437 (orange), and Pan-keratin (C11) mouse mAb #4545 (cyan).

IHC-P (paraffin)

IHC-P (paraffin)

Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using PD-L1 (E1L3N®) XP® rabbit mAb (red), CD8α (C8/144B) mouse mAb #70306 (magenta), B7-H4 (D1M8I) XP® rabbit mAb #14572 (green), FoxP3 (D2W8E™) rabbit mAb #98377 (yellow), and Pan-keratin (C11) mouse mAb #4545 (cyan).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated SUP-M2 cells using PD-L1 (E1L3N®) XP® Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.

Background

Programmed cell death 1 ligand 1 (PD-L1, B7-H1, CD274) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. The PD-L1 ligand binds the PD-1 transmembrane receptor and inhibits T cell activation. PD-L1 was discovered following a search for novel B7 protein homologs and was later shown to be expressed by antigen presenting cells, activated T cells, and tissues including placenta, heart, and lung (1-3). Similar in structure to related B7 family members, PD-L1 protein contains extracellular IgV and IgC domains and a short, cytoplasmic region. Research studies demonstrate that PD-L1 is expressed in several tumor types, including melanoma, ovary, colon, lung, breast, and renal cell carcinomas (4-6). Expression of PD-L1 in cancer is associated with tumor infiltrating lymphocytes, which mediate PD-L1 expression through the release of interferon gamma (7). Additional research links PD-L1 expression to cancers associated with viral infections (8,9).

  1. Dong, H. et al. (1999) Nat Med 5, 1365-9.
  2. Freeman, G.J. et al. (2000) J Exp Med 192, 1027-34.
  3. Liang, S.C. et al. (2003) Eur J Immunol 33, 2706-16.
  4. Dong, H. et al. (2002) Nat Med 8, 793-800.
  5. Thompson, R.H. et al. (2006) Cancer Res 66, 3381-5.
  6. Pardoll, D.M. (2012) Nat Rev Cancer 12, 252-64.
  7. Taube, J.M. et al. (2012) Sci Transl Med 4, 127ra37.
  8. Lyford-Pike, S. et al. (2013) Cancer Res 73, 1733-41.
  9. Chen, B.J. et al. (2013) Clin Cancer Res 19, 3462-73.
  10. Wimberly, H. et al. (2014) Cancer Immunol Res , .

Application References

  • Schultheis, A.M. et al. (2015) Eur J Cancer 51, 421-6. Applications: IHC-P (paraffin).
  • Cedrés, S. et al. (2015) PLoS One 10, e0121071. Applications: IHC-P (paraffin).
  • Katsuya, Y. et al. (2015) Lung Cancer 88, 154-9. Applications: IHC-P (paraffin).
  • Fang, W. et al. (2014) Oncotarget 5,12189-202. Applications: Flow Cytometry, IF-IC (In Cells), IHC-P (paraffin), Western Blotting.
  • Schmidt, L.H. et al. (2015) PLoS One 10, e0136023. Applications: IHC-P (paraffin).
  • Lastwika, K.J. et al. (2016) Cancer Res 76, 227-38. Applications: IHC-P (paraffin).
  • Wong, S.Q. et al. (2015) Cancer Res 75, 5228-34. Applications: IHC-P (paraffin).
  • Heeren, A.M. et al. (2015) Oncotarget 6, 32484-93. Applications: IHC-P (paraffin).
  • Tang, Y. et al. (2015) Oncotarget 6, 14209-19. Applications: IHC-P (paraffin).
  • Koyama, S. et al. (2016) Cancer Res 76, 999-1008. Applications: IHC-P (paraffin).
  • Koh, J. et al. (2015) Mod Pathol 28, 1154-66. Applications: IHC-P (paraffin), Western Blotting.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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