New England Biolabs Canada
 
XP Monoclonal Antibody

Product Pathways - Lymphocyte Signaling

B7-H3 (D9M2L) XP® Rabbit mAb #14058

Item# Description List Price Web Price Qty
14058S B7-H3 (D9M2L) XP® Rabbit mAb - 100 µl $433.00
$389.70
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14058T B7-H3 (D9M2L) XP® Rabbit mAb - 20 µl $184.00
$165.60
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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8059P SignalStain® DAB Substrate Kit - 1 Kit $60.00
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8059S SignalStain® DAB Substrate Kit - 1 Kit $200.00
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8112L SignalStain® Antibody Diluent - 100 ml $117.00
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8112S SignalStain® Antibody Diluent - 25 ml $57.00
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8114P SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) - 1 ml $56.00
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8114S SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) - 15 ml $525.00
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9425S RNF20 (D8C2) Rabbit mAb - 100 µl $372.00
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9997S Tris Buffered Saline with Tween® 20 (TBST-10X) - 1000 ml $90.00
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9998S BSA - 50 g $313.00
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human Endogenous 90 Rabbit IgG
IHC-Bond
IHC-P

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-Bond=IHC-Leica® Bond™, IHC-P=Immunohistochemistry (Paraffin)

Specificity / Sensitivity

B7-H3 (D9M2L) XP® Rabbit mAb recognizes endogenous levels of total B7-H3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala94 of human B7-H3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using B7-H3 (D9M2L) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, mock transfected (-) or transfected with constructs expressing Myc-tagged full-length human PD-L1, PD-L2, B7-H3, or B7-H4 protein (as indicated), using B7-H3 (D9M2L) XP® Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IHC-Leica® Bond™

IHC-Leica® Bond™

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using B7-H3 (D9M2L) XP® Rabbit mAb performed on the Leica® BOND™ Rx.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded lung carcinoma using B7-H3 (D9M2L) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded colon carcinoma using B7-H3 (D9M2L) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded ovarian carcinoma using B7-H3 (D9M2L) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded LNCaP (left) and NCI-H69 (right) cell pellets using B7-H3 (D9M2L) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using B7-H3 (D9M2L) XP® rabbit mAb (red), PD-1 (D4W2J) XP® rabbit mAb #86163 (green), B7-H4 (D1M8I) XP® rabbit mAb (cyan), LAG3 (D2G4O™) XP® rabbit mAb #15372 (magenta), TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow), and VISTA (D1L2G™) XP® rabbit mAb #64953 (orange).

Background

B7 homolog 3 (B7-H3, CD276) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. B7-H3 protein contains two extracellular Ig-like V-type domains and two IgG-like C2-type domains, a transmembrane domain, and a short intracellular domain (1,2). Early research examining the biological process of B7-H3 suggested that B7-H3 is a positive regulator of T cell response (1). Subsequent research studies indicated that B7-H3 is a negative regulator of T cell response, and that the protein inhibits T cell proliferation (2,3). One possibility is that B7-H3 interacts with two distinct sets of receptors, resulting in seemingly opposite biological outcomes (2). B7-H3 is expressed by antigen presenting cells, activated T cells, and a few normal tissues, including placenta and prostate (1,4,5). Expression of B7-H3 is seen in several cancer types, including prostate, breast, colon, lung, and gastric cancers, and in endothelial cells from tumor associated vasculature (6-8).

  1. Chapoval, A.I. et al. (2001) Nat Immunol 2, 269-74.
  2. Leitner, J. et al. (2009) Eur J Immunol 39, 1754-64.
  3. Ling, V. et al. (2003) Genomics 82, 365-77.
  4. Petroff, M.G. et al. (2005) Am J Pathol 167, 465-73.
  5. Roth, T.J. et al. (2007) Cancer Res 67, 7893-900.
  6. Loo, D. et al. (2012) Clin Cancer Res 18, 3834-45.
  7. Seaman, S. et al. (2007) Cancer Cell 11, 539-54.
  8. Zang, X. et al. (2010) Mod Pathol 23, 1104-12.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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