New England Biolabs Canada
 
XP Monoclonal Antibody

Product Pathways - Lymphocyte Signaling

LAG3 (D2G4O™) XP® Rabbit mAb #15372

Item# Description List Price Web Price Qty
15372S LAG3 (D2G4O™) XP® Rabbit mAb - 100 µl $433.00
$389.70
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15372T LAG3 (D2G4O™) XP® Rabbit mAb - 20 µl $184.00
$165.60
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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12630P SignalFire™ Plus ECL Reagent - 20 ml $116.00
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12630S SignalFire™ Plus ECL Reagent - 100 ml $519.00
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12757P SignalFire™ Elite ECL Reagent - 20 ml $123.00
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12757S SignalFire™ Elite ECL Reagent - 100 ml $558.00
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13747S SignalSlide® PD-L1 IHC Controls - 1 Pack $200.00
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14746P SignalStain® Citrate Unmasking Solution (10X) - 25 ml $36.00
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14746S SignalStain® Citrate Unmasking Solution (10X) - 125 ml $133.00
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15019L Animal-Free Blocking Solution (5X) - 250 ml $193.00
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15019S Animal-Free Blocking Solution (5X) - 25 ml $57.00
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5425S Normal Goat Serum - 10 ml $92.00
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6883P3 SignalFire™ ECL Reagent - 50 ml $43.00
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6883S SignalFire™ ECL Reagent - 500 ml $370.00
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7074P2 Anti-rabbit IgG, HRP-linked Antibody - 100 µl $109.00
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7074S Anti-rabbit IgG, HRP-linked Antibody - 1 ml $203.00
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7074V Anti-rabbit IgG, HRP-linked Antibody - 5 ml $646.00
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7727L Biotinylated Protein Ladder Detection Pack - 3250 µl $326.00
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7727S Biotinylated Protein Ladder Detection Pack - 650 µl $92.00
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8059P SignalStain® DAB Substrate Kit - 1 Kit $60.00
$54.00
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8059S SignalStain® DAB Substrate Kit - 1 Kit $200.00
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8112L SignalStain® Antibody Diluent - 100 ml $117.00
$105.30
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8112S SignalStain® Antibody Diluent - 25 ml $57.00
$51.30
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8114P SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) - 1 ml $56.00
$50.40
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8114S SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) - 15 ml $525.00
$472.50
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9997S Tris Buffered Saline with Tween® 20 (TBST-10X) - 1000 ml $90.00
$81.00
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9998S BSA - 50 g $313.00
$281.70
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human Endogenous 60-80 Rabbit IgG
IHC-Bond
IHC-P

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-Bond=IHC-Leica® Bond™, IHC-P=Immunohistochemistry (Paraffin)

Specificity / Sensitivity

LAG3 (D2G4O™) XP® recognizes endogenous levels of total LAG3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human LAG3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HDLM-2, HuT 78, and Jurkat cells using LAG3 (D2G4O™) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IHC-Leica® Bond™

IHC-Leica® Bond™

Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma using LAG3 (D2G4O™) XP® Rabbit mAb performed on the Leica® Bond™ Rx.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HDLM-2 (left) and PC-3 (right) cell pellets on SignalSlide® PD-L1 IHC Controls #13747 using LAG3 (D2G4O™) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colitis using LAG3 (D2G4O™) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using LAG3 (D2G4O™) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Multiplex immunohistochemical analysis of paraffin-embedded human breast carcinoma usng LAG3 (D2G4O™) XP® rabbit mAb (magenta), PD-1 (D4W2J) XP® rabbit mAb #86163 (green), PD-L1 (E1L3N®) XP® rabbit mAb #13684 (red), TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow), CD8α (C8/144B) mouse mAb #70306 (orange), and Pan-keratin (C11) mouse mAb #4545 (cyan).


IHC-P (paraffin)

IHC-P (paraffin)

Multiplex immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using LAG3 (D2G4O™) XP® rabbit mAb (orange), PD-1 (EH33) mouse mAb #43248 (green), CD8α (C8/144B) mouse mAb #70306 (magenta), CD68 (D4B9C) XP® rabbit mAb #76437 (red), Pan-keratin (C11) mouse mAb #4545 (cyan), and TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow).

IHC-P (paraffin)

IHC-P (paraffin)

Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using LAG3 (D2G4O™) XP® rabbit mAb (magenta), PD-1 (D4W2J) XP® rabbit mAb #86163 (green), B7-H3 (D9M2L) XP® rabbit mAb #14058 (red), B7-H4 (D1M8I) XP® rabbit mAb (cyan), TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow), and VISTA (D1L2G™) XP® rabbit mAb #64953 (orange).

Background

Lymphocyte activation gene 3 (LAG-3, CD223) is an immune checkpoint control protein that negatively regulates T cells and immune responses. A CD4-like member of the Ig superfamily, LAG3 contains an extracellular IgV and three IgC domains, a transmembrane domain, and a short cytoplasmic region (1). LAG3 is primarily expressed by activated CD4+ T cells, CD8+ T cells, Tregs and NK cells, where it's activated by MHC Class II molecules, its only known ligand. While it was initially shown to activate Treg cells (2), LAG3 can also inhibit CD8+ T cells (3,4). LAG3 is often co-expressed with PD-1 on the surface of tumor infiltrating lymphocytes, where the two proteins act independently to contribute to tumor-mediated immune suppression (4,5). Blockade of LAG3 is a promising strategy for neoplastic intervention (6).

  1. Triebel, F. et al. (1990) J Exp Med 171, 1393-405.
  2. Huang, C.T. et al. (2004) Immunity 21, 503-13.
  3. Grosso, J.F. et al. (2007) J Clin Invest 117, 3383-92.
  4. Woo, S.R. et al. (2012) Cancer Res 72, 917-27.
  5. Matsuzaki, J. et al. (2010) Proc Natl Acad Sci U S A 107, 7875-80.
  6. Goldberg, M.V. and Drake, C.G. (2011) Curr Top Microbiol Immunol 344, 269-78.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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