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XP Monoclonal Antibody

Product Pathways - Lineage Markers

LIN28A (D84C11) XP® Rabbit mAb #3695

Item# Description List Price Web Price Qty
3695S LIN28A (D84C11) XP® Rabbit mAb - 100 µl $432.00
$388.80
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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2840T Oct-4A (C30A3) Rabbit mAb - 20 µl $163.00
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3579S Sox2 (D6D9) XP® Rabbit mAb - 100 µl $432.00
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3579T Sox2 (D6D9) XP® Rabbit mAb - 20 µl $180.00
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3580S Nanog Antibody - 100 µl $383.00
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6883P3 SignalFire™ ECL Reagent - 50 ml $44.00
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7074S Anti-rabbit IgG, HRP-linked Antibody - 1 ml $209.00
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8112L SignalStain® Antibody Diluent - 100 ml $120.00
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8112S SignalStain® Antibody Diluent - 25 ml $59.00
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8114P SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) - 1 ml $57.00
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human Endogenous 26 Rabbit IgG
IHC-P
IF-IC
F

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry

Homology

Species predicted to react based on 100% sequence homology: Rat, Monkey.

Specificity / Sensitivity

LIN28A (D84C11) XP® Rabbit mAb detects endogenous levels of total LIN28A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acid sequence at the carboxy terminus of human LIN28A.

Western Blotting

Western Blotting

Western blot analysis of extracts of NTERA-2 and IGROV1 cells using LIN28A (D84C11) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human seminoma using LIN28A (D84C11) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tumor tissues: seminoma (top-left), prostate carcinoma (top-right), neuroendocrine lung carcinoma (bottom-left) and ductal breast carcinoma (bottom-right) using LIN28A (D84C11) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded IGROV-1 cell pellet (left, positive) or HCT 116 cell pellet (right, negative) using LIN28A (D84C11) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded normal human tissues: testis (top-left), prostate (top-right), pancreas (bottom-left) and kidney (bottom-right) using LIN28A (D84C11) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cells using Lin28A (D84C11) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using LIN28A (D84C11) XP® Rabbit mAb.

Background

LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7).

  1. Balzer, E. and Moss, E.G. (2007) RNA Biol 4, 16-25.
  2. Piskounova, E. et al. (2008) J Biol Chem 283, 21310-4.
  3. Cho, W.C. (2007) Mol Cancer 6, 60.
  4. Viswanathan, S.R. et al. (2009) Nat Genet 41, 843-8.
  5. West, J.A. et al. (2009) Nature 460, 909-13.
  6. Ong, K.K. et al. (2009) Nat Genet 41, 729-33.
  7. Yu, J. et al. (2007) Science 318, 1917-20.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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