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Product Pathways - Cytoskeletal Signaling

β-Actin (13E5) Rabbit mAb (HRP Conjugate) #5125

Item# Description List Price Web Price Qty
5125S β-Actin (13E5) Rabbit mAb (HRP Conjugate) - 100 µl $436.00
$392.40
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse, Rat, Monkey, Bovine, Pig Endogenous 45 Rabbit IgG

Species cross-reactivity is determined by western blot using the unconjugated antibody.

Applications Key: W=Western Blotting

Homology

Species predicted to react based on 100% sequence homology: Hamster, Chicken, Dog, Horse.

Protocols

Specificity / Sensitivity

β-Actin (13E5) Rabbit mAb (HRP Conjugate) detects endogenous levels of total β-actin protein. This antibody may cross-react with γ-actin (cytoplasmic isoform). It does not cross-react with α-skeletal, α-cardiac, α-vascular smooth muscle, or γ-enteric smooth muscle isoforms.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding residues near the amino terminus of human β-actin.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using β-Actin (13E5) Rabbit mAb (HRP Conjugate).

Description

This Cell Signaling Technology (CST) antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody (β-Actin (13E5) Rabbit mAb #4970).

Background

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (6).

Application References

  • Xiang, X. et al. (2011) PLoS One 6, e14640. Applications: Western Blotting.
  • Pulikkan, J.A. et al. (2012) Blood , . Applications: Western Blotting.
  • Haaß, W. et al. (2012) PLoS One 7, e42863. Applications: Western Blotting.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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