New England Biolabs Canada
 
XP Monoclonal Antibody

Product Pathways - Immunohistochemistry

VISTA (D5L5T) XP® Rabbit mAb #54979

Item# Description List Price Web Price Qty
54979S VISTA (D5L5T) XP® Rabbit mAb - 100 µl $433.00
$389.70
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54979T VISTA (D5L5T) XP® Rabbit mAb - 20 µl $184.00
$165.60
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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8114P SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) - 1 ml $56.00
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8114S SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) - 15 ml $525.00
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse, Rat Endogenous 45-65 Rabbit IgG
IP
IHC-Bond
IHC-P
IF-F
IF-IC

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-Bond=IHC-Leica® Bond™, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry)

Specificity / Sensitivity

VISTA (D5L5T) XP® Rabbit mAb recognizes endogenous levels of total VISTA protein. This antibody is not recommended for immunohistochemical analysis of human tissues. Instead, VISTA (D1L2G™) XP® Rabbit mAb #64953 is suggested.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val291 of mouse VISTA protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of Raw 264.7 cells (left, positive) and HeLa cells (right, negative) using VISTA (D5L5T) XP® Rabbit mAb (green) and a β-Actin Mouse mAb (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and tissues using VISTA (D5L5T) XP® Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of VISTA protein from EL4 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is VISTA (D5L5T) XP® Rabbit mAb. Western blot analysis was performed using VISTA (D5L5T) XP® Rabbit mAb.


IHC-Leica® Bond™

IHC-Leica® Bond™

Immunohistochemical analysis of paraffin-embedded mouse CT26 syngeneic tumor using VISTA (D5L5T) XP® Rabbit mAb performed on the Leica® Bond™ Rx.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse colon using VISTA (D5L5T) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse lung using VISTA (D5L5T) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded EL4 cell pellet (left, positive) or 3T3 cell pellet (right, negative) using VISTA (D5L5T) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse spleen using VISTA (D5L5T) XP® Rabbit mAb.

IF-F

IF-F

Confocal immunofluorescent analysis of mouse colon using VISTA (D5L5T) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).


Background

VISTA (V-Domain Ig Suppressor of T Cell Activation) is a negative checkpoint control protein that regulates T cell activation and immune responses. VISTA, which contains a single Ig-like V-type domain, a transmembrane domain, and an intracellular domain, has sequence similarity to both the B7 and CD28 family members. Although primarily expressed by myeloid cells, VISTA is also expressed by CD4+, CD8+, and FoxP3+ T-cells. Thus, VISTA is described as both a ligand and a receptor (1-3). Blocking VISTA induces T-cell activation and proliferation, and potentiates disease severity in the EAE model (1). Furthermore, genetic deletion of VISTA in mice leads to spontaneous T-cell activation and chronic inflammation (4,5). In mouse models of cancer, neutralization of VISTA enhances T-cell proliferation and effector function and increases tumor infiltration, suggesting VISTA blockade could be an effective strategy for tumor immunotherapy (6,7).

  1. Wang, L. et al. (2011) J Exp Med 208, 577-92.
  2. Flies, D.B. et al. (2011) J Immunol 187, 1537-41.
  3. Lines, J.L. et al. (2014) Cancer Res 74, 1924-32.
  4. Wang, L. et al. (2014) Proc Natl Acad Sci U S A 111, 14846-51.
  5. Liu, J. et al. (2015) Proc Natl Acad Sci U S A 112, 6682-7.
  6. Le Mercier, I. et al. (2014) Cancer Res 74, 1933-44.
  7. Lines, J.L. et al. (2014) Cancer Immunol Res 2, 510-7.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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