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Product Pathways - Apoptosis

Gasdermin B Antibody #76439

Item# Description List Price Web Price Qty
76439S Gasdermin B Antibody - 100 µl $372.00
$334.80
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Source
W Human Endogenous 47 Rabbit
IP

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation

Specificity / Sensitivity

Gasdermin B Antibody recognizes endogenous levels of total Gasdermin B protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu96 of human Gasdermin B protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Gasdermin B (hGSDMB-Myc/DDK; +), using Gasdermin B Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Gasdermin B Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of Gasdermin B from COLO 205 extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 Gasdermin B Antibody. Western blot was performed using Gasdermin B Antibody. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.


Background

The gasdermin family that includes GSDMA, GSDMB, GSMDC, GSDMD, and GSDME have been shown to play a role in inflammation and cell death. Gasedermin D has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11/4/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSMDM-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7).

Gasdermin B (GSDMB) has been reported to be upregulated in several tumor types, and in breast cancer has been associated with metastasis and poor prognosis (8,9). In addition Gasdermin B has been associated with immune disorders including asthma (10,11). Gasdermin B expression has also be found in the lung epithelium associated with asthma. Gasdermin B can also have a role in pyroptosis as it was found to activate caspase-4 and promote Gasdermin D cleavage (12).

  1. Kayagaki, N. et al. (2015) Nature 526, 666-71.
  2. Shi, J. et al. (2015) Nature 526, 660-5.
  3. Broz, P. and Dixit, V.M. (2016) Nat Rev Immunol 16, 407-20.
  4. Aglietti, R.A. et al. (2016) Proc Natl Acad Sci U S A 113, 7858-63.
  5. Ding, J. et al. (2016) Nature 535, 111-6.
  6. Liu, X. et al. (2016) Nature 535, 153-8.
  7. Sborgi, L. et al. (2016) EMBO J 35, 1766-78.
  8. Hergueta-Redondo, M. et al. (2014) PLoS One 9, e90099.
  9. Hergueta-Redondo, M. et al. (2016) Oncotarget 7, 56295-308.
  10. Yu, J. et al. (2011) Pediatr Pulmonol 46, 701-8.
  11. Das, S. et al. (2016) Proc Natl Acad Sci U S A 113, 13132-7.
  12. Chen, Q. et al. (2018) J Mol Cell Biol , in press.

Application References

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