New England Biolabs Canada

Product Pathways - Apoptosis

Gasdermin B Antibody #76439

Item# Description List Price Web Price Qty
76439S Gasdermin B Antibody - 100 µl $372.00
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at
Application Dilution Species-Reactivity Sensitivity MW (kDa) Source
W Human Endogenous 47 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation

Specificity / Sensitivity

Gasdermin B Antibody recognizes endogenous levels of total Gasdermin B protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu96 of human Gasdermin B protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Gasdermin B (hGSDMB-Myc/DDK; +), using Gasdermin B Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Gasdermin B Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).



Immunoprecipitation of Gasdermin B from COLO 205 extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 Gasdermin B Antibody. Western blot was performed using Gasdermin B Antibody. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.


The gasdermin family that includes GSDMA, GSDMB, GSMDC, GSDMD, and GSDME have been shown to play a role in inflammation and cell death. Gasedermin D has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11/4/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSMDM-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7).

Gasdermin B (GSDMB) has been reported to be upregulated in several tumor types, and in breast cancer has been associated with metastasis and poor prognosis (8,9). In addition Gasdermin B has been associated with immune disorders including asthma (10,11). Gasdermin B expression has also be found in the lung epithelium associated with asthma. Gasdermin B can also have a role in pyroptosis as it was found to activate caspase-4 and promote Gasdermin D cleavage (12).

  1. Kayagaki, N. et al. (2015) Nature 526, 666-71.
  2. Shi, J. et al. (2015) Nature 526, 660-5.
  3. Broz, P. and Dixit, V.M. (2016) Nat Rev Immunol 16, 407-20.
  4. Aglietti, R.A. et al. (2016) Proc Natl Acad Sci U S A 113, 7858-63.
  5. Ding, J. et al. (2016) Nature 535, 111-6.
  6. Liu, X. et al. (2016) Nature 535, 153-8.
  7. Sborgi, L. et al. (2016) EMBO J 35, 1766-78.
  8. Hergueta-Redondo, M. et al. (2014) PLoS One 9, e90099.
  9. Hergueta-Redondo, M. et al. (2016) Oncotarget 7, 56295-308.
  10. Yu, J. et al. (2011) Pediatr Pulmonol 46, 701-8.
  11. Das, S. et al. (2016) Proc Natl Acad Sci U S A 113, 13132-7.
  12. Chen, Q. et al. (2018) J Mol Cell Biol , in press.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Toll Free: 1-800-387-1095

Fax: 1-800-563-3789

About NEBTerms of Sale Web Discount Shipping Information Web Site Disclaimer Privacy Policy NEB USA Cell Signaling Technology Sitemap
Contents ¬©New England Biolabs Ltd.  New England Biolabs Ltd. is the exclusive Canadian distributor for Cell Signaling Technology, Inc.  New England Biolabs, Inc. is an ISO 9001 certified company.

Search another product:


Item has been added to the cart


Item has been added to the favourites