New England Biolabs Canada
 
XP Monoclonal Antibody

Product Pathways - Neuroscience

GFAP (E4L7M) XP® Rabbit mAb #80788

Item# Description List Price Web Price Qty
80788S GFAP (E4L7M) XP® Rabbit mAb - 100 µl $424.00
$381.60
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80788T GFAP (E4L7M) XP® Rabbit mAb - 20 µl $181.00
$162.90
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

Item# Description List Price Web Price Qty
12389S GFAP (D1F4Q) XP® Rabbit mAb - 100 µl $410.00
$369.00
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12389T GFAP (D1F4Q) XP® Rabbit mAb - 20 µl $171.00
$153.90
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12630P SignalFire™ Plus ECL Reagent - 20 ml $113.00
$101.70
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12630S SignalFire™ Plus ECL Reagent - 100 ml $508.00
$457.20
$304.80
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12757P SignalFire™ Elite ECL Reagent - 20 ml $120.00
$108.00
$72.00
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12757S SignalFire™ Elite ECL Reagent - 100 ml $546.00
$491.40
$327.60
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12957S Western Blotting Application Solutions Kit - 1 Kit $587.00
$528.30
$440.25
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14746P SignalStain® Citrate Unmasking Solution (10X) - 25 ml $35.00
$31.50
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14746S SignalStain® Citrate Unmasking Solution (10X) - 125 ml $130.00
$117.00
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15019L Animal-Free Blocking Solution (5X) - 250 ml $189.00
$170.10
$141.75
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15019S Animal-Free Blocking Solution (5X) - 25 ml $56.00
$50.40
$42.00
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3670S GFAP (GA5) Mouse mAb - 100 µl $364.00
$327.60
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3670T GFAP (GA5) Mouse mAb - 20 µl $155.00
$139.50
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47084S GFAP (D1F4Q) XP® Rabbit mAb (Biotinylated) - 100 µl $487.00
$438.30
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5142T Loading Control Antibody Sampler Kit - 1 Kit $532.00
$478.80
$399.00
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5425S Normal Goat Serum - 10 ml $90.00
$81.00
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6883P3 SignalFire™ ECL Reagent - 50 ml $42.00
$37.80
$25.20
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6883S SignalFire™ ECL Reagent - 500 ml $363.00
$326.70
$217.80
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7003P 20X LumiGLO® Reagent and 20X Peroxide - 5 ml each $90.00
$81.00
$54.00
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7003S 20X LumiGLO® Reagent and 20X Peroxide - 25 ml each $287.00
$258.30
$172.20
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7071S Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody - 50 assays $395.00
$355.50
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7072S Phototope-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody - 50 assays $395.00
$355.50
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7074P2 Anti-rabbit IgG, HRP-linked Antibody - 100 µl $106.00
$95.40
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7074S Anti-rabbit IgG, HRP-linked Antibody - 1 ml $199.00
$179.10
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7074V Anti-rabbit IgG, HRP-linked Antibody - 5 ml $633.00
$569.70
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7075S Anti-biotin, HRP-linked Antibody - 1 ml $189.00
$170.10
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7727L Biotinylated Protein Ladder Detection Pack - 3250 µl $319.00
$287.10
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7727S Biotinylated Protein Ladder Detection Pack - 650 µl $90.00
$81.00
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8059P SignalStain® DAB Substrate Kit - 1 Kit $59.00
$53.10
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8059S SignalStain® DAB Substrate Kit - 1 Kit $196.00
$176.40
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8112L SignalStain® Antibody Diluent - 100 ml $115.00
$103.50
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8112S SignalStain® Antibody Diluent - 25 ml $56.00
$50.40
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8114P SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) - 1 ml $55.00
$49.50
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8114S SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) - 15 ml $514.00
$462.60
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9997S Tris Buffered Saline with Tween® 20 (TBST-10X) - 1000 ml $88.00
$79.20
$66.00
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9998S BSA - 50 g $307.00
$276.30
$230.25
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9999S Nonfat Dry Milk - 250 g $84.00
$75.60
$63.00
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse, Rat Endogenous 50 Rabbit IgG
IHC-P
IF-F

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen)

Specificity / Sensitivity

GFAP (E4L7M) XP® Rabbit mAb recognizes endogenous levels of total GFAP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human GFAP protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various tissues and cell lines using GFAP (E4L7M) XP® Rabbit mAb and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of various paraffin-embedded normal mouse tissues: brain cortex (top-left), colon (top-center), small intestine (top-right), liver (bottom-left), prostate (bottom-center) and skeletal muscle (bottom-right) using GFAP (E4L7M) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human cerebellum using GFAP (E4L7M) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human glioblastoma using GFAP (E4L7M) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human medulloblastoma using GFAP (E4L7M) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse hippocampus using GFAP (E4L7M) XP® Rabbit mAb (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded rat brain, cortex (left) and cerebellum (right), using GFAP (E4L7M) XP® Rabbit mAb.

IF-F

IF-F

Confocal immunofluorescent analysis of adult mouse cerebellum (left) and hippocampus (right) using GFAP (E4L7M) XP® Rabbit mAb (green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Background

The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are specifically expressed in particular cell types: cytokeratins in epithelial cells, glial fibrillary acidic protein (GFAP) in glial cells, desmin in skeletal, visceral, and certain vascular smooth muscle cells, vimentin in cells of mesenchymal origin, and neurofilaments in neurons. GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). In addition, GFAP intermediate filaments are also present in nonmyelin-forming Schwann cells in the peripheral nervous system (3).

  1. Eng, L.F. et al. (2000) Neurochem. Res. 25, 1439-51.
  2. Goebel, H.H. et al. (1987) Acta. Histochem. Suppl. 34, 81-93.
  3. Jessen, K.R. et al. (1990) Development 109, 91-103.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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