New England Biolabs Canada
 

Product Pathways - Motif Antibodies

Acetylated-Lysine Antibody #9441

Item# Description List Price Web Price Qty
9441L Acetylated-Lysine Antibody - 300 µl $1,025.00
$922.50
ADD TO CART
9441S Acetylated-Lysine Antibody - 100 µl $433.00
$389.70
ADD TO CART
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
X

Companion Products

Item# Description List Price Web Price Qty
12630P SignalFire™ Plus ECL Reagent - 20 ml $116.00
$104.40
ADD TO CART
12630S SignalFire™ Plus ECL Reagent - 100 ml $519.00
$467.10
ADD TO CART
12757P SignalFire™ Elite ECL Reagent - 20 ml $123.00
$110.70
ADD TO CART
12757S SignalFire™ Elite ECL Reagent - 100 ml $558.00
$502.20
ADD TO CART
14746P SignalStain® Citrate Unmasking Solution (10X) - 25 ml $36.00
$32.40
ADD TO CART
14746S SignalStain® Citrate Unmasking Solution (10X) - 125 ml $133.00
$119.70
ADD TO CART
15019L Animal-Free Blocking Solution (5X) - 250 ml $193.00
$173.70
ADD TO CART
15019S Animal-Free Blocking Solution (5X) - 25 ml $57.00
$51.30
ADD TO CART
2062S Histone Deacetylase 1 (HDAC1) Antibody - 100 µl $372.00
$334.80
ADD TO CART
2072S Histone Deacetylase 4 (HDAC4) Antibody - 100 µl $372.00
$334.80
ADD TO CART
2152S WSTF Antibody - 100 µl $372.00
$334.80
ADD TO CART
5425S Normal Goat Serum - 10 ml $92.00
$82.80
ADD TO CART
6883P3 SignalFire™ ECL Reagent - 50 ml $43.00
$38.70
ADD TO CART
6883S SignalFire™ ECL Reagent - 500 ml $370.00
$333.00
ADD TO CART
7074P2 Anti-rabbit IgG, HRP-linked Antibody - 100 µl $109.00
$98.10
ADD TO CART
7074S Anti-rabbit IgG, HRP-linked Antibody - 1 ml $203.00
$182.70
ADD TO CART
7074V Anti-rabbit IgG, HRP-linked Antibody - 5 ml $646.00
$581.40
ADD TO CART
7727L Biotinylated Protein Ladder Detection Pack - 3250 µl $326.00
$293.40
ADD TO CART
7727S Biotinylated Protein Ladder Detection Pack - 650 µl $92.00
$82.80
ADD TO CART
8059P SignalStain® DAB Substrate Kit - 1 Kit $60.00
$54.00
ADD TO CART
8059S SignalStain® DAB Substrate Kit - 1 Kit $200.00
$180.00
ADD TO CART
9681S Acetylated-Lysine Mouse mAb (Ac-K-103) - 400 µl $355.00
$319.50
ADD TO CART
9701L Phospho-Histone H3 (Ser10) Antibody - 300 µl $1,025.00
$922.50
ADD TO CART
9701S Phospho-Histone H3 (Ser10) Antibody - 100 µl $433.00
$389.70
ADD TO CART
9706L Phospho-Histone H3 (Ser10) (6G3) Mouse mAb - 300 µl $1,025.00
$922.50
ADD TO CART
9706S Phospho-Histone H3 (Ser10) (6G3) Mouse mAb - 100 µl $433.00
$389.70
ADD TO CART
9711S Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody - 100 µl $433.00
$389.70
ADD TO CART
9715L Histone H3 Antibody - 300 µl $901.00
$810.90
ADD TO CART
9715S Histone H3 Antibody - 100 µl $372.00
$334.80
ADD TO CART
9997S Tris Buffered Saline with Tween® 20 (TBST-10X) - 1000 ml $90.00
$81.00
ADD TO CART
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity Source
W All Species Expected Endogenous Rabbit
IP
IHC-P
IF-IC
ChIP
E-P

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP, E-P=Peptide ELISA (DELFIA)

Directions For Use

For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

Specificity / Sensitivity

Acetylated-Lysine Antibody detects proteins posttranslationally modified by acetylation on the epsilon-amine groups of lysine residues. The antibody recognizes acetylated lysine in a wide range of sequence contexts. It has been demonstrated to recognize acetylated histones, p53, CBP, PCAF and chemically acetylated BSA. The antibody has been shown to react with as little as 0.04 ng of chemically acetylated BSA while not recognizing up to 25 µg of nonacetylated BSA. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic acetylated lysine-containing peptide. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or sodium butyrate-treated (5 mM for 24 hours), showing an increase in histone acetylation using Acetylated-Lysine Antibody.

Western Blotting

Western Blotting

Specificity and sensitivity of Acetylated-Lysine Antibody assayed on acetylated BSA (4; 1; 0.2; 0.04 or 0.008 ng in lanes 1-5) or nonacetylated BSA (25,000; 5,000; 1,000 or 200 ng in lanes 6-9).

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untreated or TSA-treated, grown in 10% FBS (lanes 1 and 2) or serum starved for 18 hours (lanes 3 and 4), using Acetylated-Lysine Antibody (upper) or p44/42 MAP Kinase Antibody #9102 (lower).


IP

IP

Western blot analysis of immunoprecipitated p53 showing an increase in p53 acetylation using Acetylated-Lysine Antibody (upper) or p53 antibody (lower). p53 was immunoprecipitated from lysates from 293 cells, untreated or UV-treated, using p53 Antibody #9282.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Acetylated-Lysine Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical staining of a paraffin-embedded human breast tumor section showing nuclear and cytoplasmic localization of proteins with acetylated lysine residues using Acetylated-Lysine Antibody.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded NIH/3T3 untreated (left) or TSA-treated (right) using Acetylated-Lysine Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NIH/3T3 cells, untreated (left) or SAHA-treated (right), labeled with Acetylated-Lysine Antibody (green). Actin filaments have been labeled with Alexa Fluor R 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Acetylated-Lysine Antibody or Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads) #9002. The enriched DNA was quantified by real-time PCR, using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human MYT-1 Exon 1 Primers #4493. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


Background

Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (1). Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis (2-6). Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of posttranslational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport (7,8). The regulation of protein acetylation status is impaired in cancer and polyglutamine diseases (9), and HDACs have become promising targets for anti-cancer drugs currently in development (10).

  1. Hassig, C.A. and Schreiber, S.L. (1997) Curr Opin Chem Biol 1, 300-8.
  2. Allfrey, V.G. et al. (1964) Proc Natl Acad Sci USA 51, 786-94.
  3. Liu, L. et al. (1999) Mol Cell Biol 19, 1202-9.
  4. Boyes, J. et al. (1998) Nature 396, 594-8.
  5. Polevoda, B. and Sherman, F. (2002) Genome Biol 3, reviews 0006.
  6. Yoshida, M. et al. (2003) Prog Cell Cycle Res 5, 269-78.
  7. Kim, S.C. et al. (2006) Mol Cell 23, 607-18.
  8. Choudhary, C. et al. (2009) Science 325, 834-40.
  9. Hughes, R.E. (2002) Curr Biol 12, R141-3.
  10. Vigushin, D.M. and Coombes, R.C. (2004) Curr Cancer Drug Targets 4, 205-18.

Application References

  • Werner, H.B. et al. (2007) J Neurosci 27, 7717-30. Applications: Western Blotting.
  • Yuan, Z.L. et al. (2005) Science 307, 269-73. Applications: Western Blotting.
  • Hu, J. and Colburn, N.H. (2005) Mol Cancer Res 3, 100-9. Applications: Western Blotting.
  • Kurahashi, T. et al. (2005) Mol Biol Cell 16, 4705-13. Applications: Western Blotting.
  • Wang, R. et al. (2005) J Biol Chem 280, 11528-34. Applications: Western Blotting.
  • Chen, L. et al. (2005) Mol Cancer Ther 4, 1311-9. Applications: Western Blotting.
  • Zheng, G. and Yang, Y.C. (2005) J Biol Chem 280, 40773-81. Applications: Western Blotting.
  • Qiu, Y. et al. (2004) J Biol Chem 279, 9796-802. Applications: IP.
  • Ard, P.G. et al. (2002) Mol Cell Biol 22, 5650-61. Applications: IP.
  • Yu, X. et al. (2002) J Natl Cancer Inst 94, 504-13. Applications: Western Blotting.
  • Bereshchenko, O.R. et al. (2002) Nat Genet 32, 606-13. Applications: Western Blotting.
  • Chen Lf et al. (2001) Science 293, 1653-7. Applications: Western Blotting.
  • Sano, Y. and Ishii, S. (2001) J Biol Chem 276, 3674-82. Applications: Western Blotting.
  • Fu, M. et al. (2000) J Biol Chem 275, 20853-60. Applications: IP.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

Toll Free: 1-800-387-1095

Fax: 1-800-563-3789

info.ca@neb.com

orders.ca@neb.com

About NEBTerms of Sale Web Discount Shipping Information Web Site Disclaimer Privacy Policy NEB USA Cell Signaling Technology Sitemap
Contents ¬©New England Biolabs Ltd.  New England Biolabs Ltd. is the exclusive Canadian distributor for Cell Signaling Technology, Inc.  New England Biolabs, Inc. is an ISO 9001 certified company.

Search another product:

 

Item has been added to the cart

 

Item has been added to the favourites