New England Biolabs Canada
 

Product Pathways - Protein Translation

4E-BP1 (53H11) Rabbit mAb #9644

Item# Description List Price Web Price Qty
9644S 4E-BP1 (53H11) Rabbit mAb - 100 µl $383.00
$344.70
ADD TO CART
9644T 4E-BP1 (53H11) Rabbit mAb - 20 µl $163.00
$146.70
ADD TO CART
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
X

Companion Products

Item# Description List Price Web Price Qty
1053S 4E-BP1 Blocking Peptide - 100 µg $472.00
$424.80
ADD TO CART
12630P SignalFire™ Plus ECL Reagent - 20 ml $120.00
$108.00
ADD TO CART
12630S SignalFire™ Plus ECL Reagent - 100 ml $519.00
$467.10
ADD TO CART
12757P SignalFire™ Elite ECL Reagent - 20 ml $127.00
$114.30
ADD TO CART
12757S SignalFire™ Elite ECL Reagent - 100 ml $558.00
$502.20
ADD TO CART
13396S Phospho-4E-BP1 (Thr70) (D7F6I) Rabbit mAb - 100 µl $446.00
$401.40
ADD TO CART
13443S Phospho-4E-BP1 (Ser65) (D9G1Q) Rabbit mAb - 100 µl $446.00
$401.40
ADD TO CART
2845S 4E-BP2 Antibody - 100 µl $383.00
$344.70
ADD TO CART
2845T 4E-BP2 Antibody - 20 µl $163.00
$146.70
ADD TO CART
2855L Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb - 300 µl $1,057.00
$951.30
ADD TO CART
2855S Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb - 100 µl $446.00
$401.40
ADD TO CART
2855T Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb - 20 µl $180.00
$162.00
ADD TO CART
4923S Non-phospho-4E-BP1 (Thr46) (87D12) Rabbit mAb - 100 µl $446.00
$401.40
ADD TO CART
4923T Non-phospho-4E-BP1 (Thr46) (87D12) Rabbit mAb - 20 µl $180.00
$162.00
ADD TO CART
6883P3 SignalFire™ ECL Reagent - 50 ml $44.00
$39.60
ADD TO CART
6883S SignalFire™ ECL Reagent - 500 ml $382.00
$343.80
ADD TO CART
7074P2 Anti-rabbit IgG, HRP-linked Antibody - 100 µl $112.00
$100.80
ADD TO CART
7074S Anti-rabbit IgG, HRP-linked Antibody - 1 ml $209.00
$188.10
ADD TO CART
7074V Anti-rabbit IgG, HRP-linked Antibody - 5 ml $672.00
$604.80
ADD TO CART
7216C PathScan® Phospho-4E-BP1 (Thr37/Thr46) Sandwich ELISA Kit - 1 Kit $714.00
$642.60
ADD TO CART
7727L Biotinylated Protein Ladder Detection Pack - 3250 µl $336.00
$302.40
ADD TO CART
7727S Biotinylated Protein Ladder Detection Pack - 650 µl $94.00
$84.60
ADD TO CART
8101S SignalSlide® Phospho-Akt (Ser473) IHC Controls - 1 Pack $206.00
$185.40
ADD TO CART
8112L SignalStain® Antibody Diluent - 100 ml $120.00
$108.00
ADD TO CART
8112S SignalStain® Antibody Diluent - 25 ml $59.00
$53.10
ADD TO CART
9451L Phospho-4E-BP1 (Ser65) Antibody - 300 µl $1,057.00
$951.30
ADD TO CART
9451S Phospho-4E-BP1 (Ser65) Antibody - 100 µl $446.00
$401.40
ADD TO CART
9451T Phospho-4E-BP1 (Ser65) Antibody - 20 µl $180.00
$162.00
ADD TO CART
9452L 4E-BP1 Antibody - 300 µl $928.00
$835.20
ADD TO CART
9452S 4E-BP1 Antibody - 100 µl $383.00
$344.70
ADD TO CART
9455L Phospho-4E-BP1 (Thr70) Antibody - 300 µl $1,057.00
$951.30
ADD TO CART
9455S Phospho-4E-BP1 (Thr70) Antibody - 100 µl $446.00
$401.40
ADD TO CART
9456S Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb - 100 µl $446.00
$401.40
ADD TO CART
9459L Phospho-4E-BP1 (Thr37/46) Antibody - 300 µl $1,057.00
$951.30
ADD TO CART
9459S Phospho-4E-BP1 (Thr37/46) Antibody - 100 µl $446.00
$401.40
ADD TO CART
9741L Phospho-eIF4E (Ser209) Antibody - 300 µl $1,057.00
$951.30
ADD TO CART
9741S Phospho-eIF4E (Ser209) Antibody - 100 µl $446.00
$401.40
ADD TO CART
9741T Phospho-eIF4E (Ser209) Antibody - 20 µl $180.00
$162.00
ADD TO CART
9918T Translational Control Antibody Sampler Kit - 1 Kit $636.00
$572.40
ADD TO CART
9955T 4E-BP Antibody Sampler Kit - 1 Kit $636.00
$572.40
ADD TO CART
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse, Rat, Monkey Endogenous 15-20 Rabbit IgG
IP
IHC-P
IF-IC
F

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry

Specificity / Sensitivity

4E-BP1 (53H11) Rabbit mAb detects endogenous levels of total 4E-BP1 protein.

Source / Purification

4E-BP1 (53H11) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Ser112 of human 4E-BP1.

Western Blotting

Western Blotting

Western blot analysis of HeLa cell extracts, untreated (-) or 4E-BP1 knock-out (+), using 4E-BP1 (53H11) Rabbit mAb #9644 (upper) or β-actin (13E5) Rabbit mAb #4970 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using 4E-BP1 (53H11) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using 4E-BP1 (53H11) Rabbit mAb in the presence of control peptide (left) or 4E-BP1 blocking peptide #1053 (right).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using 4E-BP1 (53H11) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using 4E-BP1 (53H11) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse spleen, 4E-BP1/2 wild type (left) or 4E-BP1 knockout (right), using 4E-BP1 (53H11) Rabbit mAb. 4E-BP wild type and knockout tissues kindly provided by Dr. Nahum Sonenberg, McGill University.


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using 4E-BP1 (53H11) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of 293 cells, expressing either nontargeting shRNA (left) or shRNA targeting 4E-BP1/2 (right), using 4E-BP1 (53H11) Rabbit mAb #9644 (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4084.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of COS cells using 4E-BP1 (53H11) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).


Background

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).

  1. Pause, A. et al. (1994) Nature 371, 762-7.
  2. Brunn, G.J. et al. (1997) Science 277, 99-101.
  3. Gingras, A.C. et al. (1998) Genes Dev 12, 502-13.
  4. Fadden, P. et al. (1997) J Biol Chem 272, 10240-7.
  5. Gingras, A.C. et al. (1999) Genes Dev 13, 1422-37.

Application References

  • Rong, L. et al. (2008) RNA 14, 1318-27. Applications: IHC-P (paraffin).
  • Gómez-Abad, C. et al. (2011) Blood 118, 5517-27. Applications: Western Blotting.
  • Roczniak-Ferguson, A. et al. (2012) Sci Signal 5, ra42. Applications: Western Blotting.
  • Tagoug, I. et al. (2011) PLoS One 6, e22641. Applications: Western Blotting.
  • Murai, A. et al. (2012) BMC Vet Res 8, 128. Applications: Western Blotting.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

Toll Free: 1-800-387-1095

Fax: 1-800-563-3789

info.ca@neb.com

orders.ca@neb.com

About NEBTerms of Sale Web Discount Shipping Information Web Site Disclaimer Privacy Policy NEB USA Cell Signaling Technology Sitemap
Contents ¬©New England Biolabs Ltd.  New England Biolabs Ltd. is the exclusive Canadian distributor for Cell Signaling Technology, Inc.  New England Biolabs, Inc. is an ISO 9001 certified company.

Search another product:

 

Item has been added to the cart

 

Item has been added to the favourites