New England Biolabs Canada
 

Product Pathways - Chromatin Regulation / Epigenetics

Acetyl-Histone H3 (Lys18) Antibody #9675

Item# Description List Price Web Price Qty
9675S Acetyl-Histone H3 (Lys18) Antibody - 100 µl $433.00
$389.70
ADD TO CART
9675T Acetyl-Histone H3 (Lys18) Antibody - 20 µl $174.00
$156.60
ADD TO CART
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
X

Companion Products

Item# Description List Price Web Price Qty
12630P SignalFire™ Plus ECL Reagent - 20 ml $116.00
$104.40
ADD TO CART
12630S SignalFire™ Plus ECL Reagent - 100 ml $519.00
$467.10
ADD TO CART
12757P SignalFire™ Elite ECL Reagent - 20 ml $123.00
$110.70
ADD TO CART
12757S SignalFire™ Elite ECL Reagent - 100 ml $558.00
$502.20
ADD TO CART
2650S Histone H3 Antibody (ChIP Formulated) - 100 µl $372.00
$334.80
ADD TO CART
2751S Phospho-Lck (Tyr505) Antibody - 100 µl $433.00
$389.70
ADD TO CART
2751T Phospho-Lck (Tyr505) Antibody - 20 µl $174.00
$99.00
ADD TO CART
4499L Histone H3 (D1H2) XP® Rabbit mAb - 300 µl $994.00
$894.60
ADD TO CART
4499S Histone H3 (D1H2) XP® Rabbit mAb - 100 µl $419.00
$377.10
ADD TO CART
4499T Histone H3 (D1H2) XP® Rabbit mAb - 20 µl $174.00
$99.00
ADD TO CART
4620S Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated) - 100 µl $419.00
$377.10
ADD TO CART
6883P3 SignalFire™ ECL Reagent - 50 ml $43.00
$38.70
ADD TO CART
6883S SignalFire™ ECL Reagent - 500 ml $370.00
$333.00
ADD TO CART
7074P2 Anti-rabbit IgG, HRP-linked Antibody - 100 µl $109.00
$98.10
ADD TO CART
7074S Anti-rabbit IgG, HRP-linked Antibody - 1 ml $203.00
$182.70
ADD TO CART
7074V Anti-rabbit IgG, HRP-linked Antibody - 5 ml $646.00
$581.40
ADD TO CART
7727L Biotinylated Protein Ladder Detection Pack - 3250 µl $326.00
$293.40
ADD TO CART
7727S Biotinylated Protein Ladder Detection Pack - 650 µl $92.00
$82.80
ADD TO CART
8112L SignalStain® Antibody Diluent - 100 ml $117.00
$105.30
ADD TO CART
8112S SignalStain® Antibody Diluent - 25 ml $57.00
$51.30
ADD TO CART
9701L Phospho-Histone H3 (Ser10) Antibody - 300 µl $1,025.00
$922.50
ADD TO CART
9701S Phospho-Histone H3 (Ser10) Antibody - 100 µl $433.00
$389.70
ADD TO CART
9706L Phospho-Histone H3 (Ser10) (6G3) Mouse mAb - 300 µl $1,025.00
$922.50
ADD TO CART
9706S Phospho-Histone H3 (Ser10) (6G3) Mouse mAb - 100 µl $433.00
$389.70
ADD TO CART
9711S Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody - 100 µl $433.00
$389.70
ADD TO CART
9715L Histone H3 Antibody - 300 µl $901.00
$810.90
ADD TO CART
9715S Histone H3 Antibody - 100 µl $372.00
$334.80
ADD TO CART
9717S Histone H3 (3H1) Rabbit mAb - 100 µl $372.00
$334.80
ADD TO CART
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Source
W Human, Mouse, Rat Endogenous 17 Rabbit
IHC-P
ChIP

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), ChIP=Chromatin IP

Directions For Use

For optimal ChIP results, use 20 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

Specificity / Sensitivity

Acetyl-Histone H3 (Lys18) Antibody detects endogenous levels of histone H3 only when acetylated at Lys18. It does not cross-react with other acetylated histones.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys18 of human histone H3. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or TSA-treated, using Acetyl-Histone H3 (Lys18) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder showing nuclear localization using Acetyl-Histone H3 (Lys18) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells, untreated (left) or TSA-treated (right), using Acetyl-Histone H3 (Lys18) Antibody. (no counterstain)


Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Acetyl-Histone H3 (Lys18) Antibody or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Background

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

  1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
  2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
  3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
  4. Cheung, P. et al. (2000) Cell 103, 263-71.
  5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
  6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
  7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
  8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
  9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
  10. Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.
  11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
  12. Dai, B. and Rasmussen, T.P. (2007) Stem Cells 25, 2567-74.

Application References

  • Allison, S.J. and Milner, J. (2003) Cancer Res. 63, 6674-6679. Applications: Western Blotting.
  • Parsons, X. H. et al. (2003) Proc. Nat. Acad. Sci. USA 100, 1609-1614. Applications: Western Blotting.
  • Song, N. et al. (2011) Acta Histochem Cytochem 44, 183-90. Applications: IHC-P (paraffin).

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

Toll Free: 1-800-387-1095

Fax: 1-800-563-3789

info.ca@neb.com

orders.ca@neb.com

About NEBTerms of Sale Web Discount Shipping Information Web Site Disclaimer Privacy Policy NEB USA Cell Signaling Technology Sitemap
Contents ¬©New England Biolabs Ltd.  New England Biolabs Ltd. is the exclusive Canadian distributor for Cell Signaling Technology, Inc.  New England Biolabs, Inc. is an ISO 9001 certified company.

Search another product:

 

Item has been added to the cart

 

Item has been added to the favourites