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Product Pathways - Chromatin Regulation / Epigenetics

Histone H3 (3H1) Rabbit mAb #9717

Item# Description List Price Web Price Qty
9717S Histone H3 (3H1) Rabbit mAb - 100 µl $372.00
$334.80
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Companion Products

Item# Description List Price Web Price Qty
12522S Mono-Methyl-Histone H3 (Lys79) (D5X1S) Rabbit mAb - 100 µl $433.00
$389.70
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12576S Cleaved Histone H3 (Thr22) (D7J2K) Rabbit mAb - 100 µl $433.00
$389.70
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12630P SignalFire™ Plus ECL Reagent - 20 ml $116.00
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12630S SignalFire™ Plus ECL Reagent - 100 ml $519.00
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12757P SignalFire™ Elite ECL Reagent - 20 ml $123.00
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12757S SignalFire™ Elite ECL Reagent - 100 ml $558.00
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13576S Phospho-Histone H3 (Thr3) (D5G1I) Rabbit mAb - 100 µl $433.00
$389.70
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13939S Symmetric Di-Methyl Histone H3 (Arg8) (E1W5H) Rabbit mAb - 100 µl $433.00
$389.70
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13969S Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb - 100 µl $433.00
$389.70
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13969T Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb - 20 µl $167.00
$150.30
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13998S Acetyl-Histone H3 (Lys18) (D8Z5H) Rabbit mAb - 100 µl $449.00
$404.10
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14111S Mono-Methyl-Histone H3 (Lys36) (D9J1D) Rabbit mAb - 100 µl $433.00
$389.70
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14111T Mono-Methyl-Histone H3 (Lys36) (D9J1D) Rabbit mAb - 20 µl $174.00
$156.60
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14186S Mono-Methyl-Histone H3 (Lys9) (D1P5R) Rabbit mAb - 100 µl $433.00
$389.70
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14932S Acetyl-Histone H3 (Lys23) (D6Y7M) Rabbit mAb - 100 µl $433.00
$389.70
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27683S Acetyl-Histone H3 (Lys36) (D9T5Q) Rabbit mAb - 100 µl $433.00
$389.70
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2901S Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb - 100 µl $433.00
$389.70
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2901T Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb - 20 µl $174.00
$156.60
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3377S Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb - 100 µl $480.00
$432.00
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3377T Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb - 20 µl $192.00
$172.80
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4243S Acetyl-Histone H3 (Lys56) Antibody - 100 µl $433.00
$389.70
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4243T Acetyl-Histone H3 (Lys56) Antibody - 20 µl $174.00
$156.60
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4260S Tri-Methyl-Histone H3 (Lys79) Antibody - 100 µl $433.00
$389.70
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4353S Acetyl-Histone H3 (Lys27) Antibody - 100 µl $433.00
$389.70
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4353T Acetyl-Histone H3 (Lys27) Antibody - 20 µl $174.00
$156.60
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4473S Pan-Methyl-Histone H3 (Lys9) (D54) XP® Rabbit mAb - 100 µl $480.00
$432.00
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4473T Pan-Methyl-Histone H3 (Lys9) (D54) XP® Rabbit mAb - 20 µl $192.00
$172.80
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4499L Histone H3 (D1H2) XP® Rabbit mAb - 300 µl $994.00
$894.60
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4499S Histone H3 (D1H2) XP® Rabbit mAb - 100 µl $419.00
$377.10
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4499T Histone H3 (D1H2) XP® Rabbit mAb - 20 µl $174.00
$156.60
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4620S Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated) - 100 µl $419.00
$377.10
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4658S Di-Methyl-Histone H3 (Lys9) (D85B4) XP® Rabbit mAb - 100 µl $480.00
$432.00
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4658T Di-Methyl-Histone H3 (Lys9) (D85B4) XP® Rabbit mAb - 20 µl $192.00
$172.80
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4909S Tri-Methyl-Histone H3 (Lys36) (D5A7) XP® Rabbit mAb - 100 µl $480.00
$432.00
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4909T Tri-Methyl-Histone H3 (Lys36) (D5A7) XP® Rabbit mAb - 20 µl $192.00
$172.80
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5326S Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb - 100 µl $480.00
$432.00
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5326T Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb - 20 µl $192.00
$172.80
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53348S Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb - 100 µl $480.00
$432.00
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53348T Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb - 20 µl $192.00
$172.80
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5427S Di-Methyl-Histone H3 (Lys79) (D15E8) XP® Rabbit mAb - 100 µl $480.00
$432.00
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5427T Di-Methyl-Histone H3 (Lys79) (D15E8) XP® Rabbit mAb - 20 µl $192.00
$172.80
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6883P3 SignalFire™ ECL Reagent - 50 ml $43.00
$38.70
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6883S SignalFire™ ECL Reagent - 500 ml $370.00
$333.00
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7074P2 Anti-rabbit IgG, HRP-linked Antibody - 100 µl $109.00
$98.10
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7074S Anti-rabbit IgG, HRP-linked Antibody - 1 ml $203.00
$182.70
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7074V Anti-rabbit IgG, HRP-linked Antibody - 5 ml $646.00
$581.40
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74073S Tri-Methyl-Histone H3 (Lys79) (E8B3M) Rabbit mAb - 100 µl $433.00
$389.70
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7627S Acetyl-Histone H3 (Lys14) (D4B9) Rabbit mAb - 100 µl $433.00
$389.70
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7627T Acetyl-Histone H3 (Lys14) (D4B9) Rabbit mAb - 20 µl $174.00
$156.60
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7727L Biotinylated Protein Ladder Detection Pack - 3250 µl $326.00
$293.40
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7727S Biotinylated Protein Ladder Detection Pack - 650 µl $92.00
$82.80
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8173S Acetyl-Histone H3 (Lys27) (D5E4) XP® Rabbit mAb - 100 µl $480.00
$432.00
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8173T Acetyl-Histone H3 (Lys27) (D5E4) XP® Rabbit mAb - 20 µl $192.00
$172.80
ADD TO CART
84932S Mono-Methyl-Histone H3 (Lys27) (D3R8N) Rabbit mAb - 100 µl $433.00
$389.70
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9398S Mono-Methyl-Histone H3 (Lys79) Antibody - 100 µl $433.00
$389.70
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95191S Mono/Di-Methyl Histone H3 (Lys56) (D4L7L) Rabbit mAb - 100 µl $433.00
$389.70
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9649S Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb - 100 µl $433.00
$389.70
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9649T Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb - 20 µl $174.00
$156.60
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9675S Acetyl-Histone H3 (Lys18) Antibody - 100 µl $433.00
$389.70
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9675T Acetyl-Histone H3 (Lys18) Antibody - 20 µl $174.00
$156.60
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9677L Acetyl-Histone H3 (Lys9/Lys14) Antibody - 300 µl $1,025.00
$922.50
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9677S Acetyl-Histone H3 (Lys9/Lys14) Antibody - 100 µl $433.00
$389.70
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9701L Phospho-Histone H3 (Ser10) Antibody - 300 µl $1,025.00
$922.50
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9701S Phospho-Histone H3 (Ser10) Antibody - 100 µl $433.00
$389.70
ADD TO CART
9706L Phospho-Histone H3 (Ser10) (6G3) Mouse mAb - 300 µl $1,025.00
$922.50
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9706S Phospho-Histone H3 (Ser10) (6G3) Mouse mAb - 100 µl $433.00
$389.70
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9707S Methyl-Histone H3 (Arg2) Antibody - 100 µl $433.00
$389.70
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9711S Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody - 100 µl $433.00
$389.70
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9713S Phospho-Histone H3 (Ser28) Antibody - 100 µl $433.00
$389.70
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9713T Phospho-Histone H3 (Ser28) Antibody - 20 µl $174.00
$156.60
ADD TO CART
9714S Phospho-Histone H3 (Thr3) Antibody - 100 µl $433.00
$389.70
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9714T Phospho-Histone H3 (Thr3) Antibody - 20 µl $174.00
$156.60
ADD TO CART
9715L Histone H3 Antibody - 300 µl $901.00
$810.90
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9715S Histone H3 Antibody - 100 µl $372.00
$334.80
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9723S Mono-Methyl-Histone H3 (Lys4) Antibody - 100 µl $433.00
$389.70
ADD TO CART
9725S Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb - 100 µl $433.00
$389.70
ADD TO CART
9725T Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb - 20 µl $174.00
$156.60
ADD TO CART
9727L Tri-Methyl-Histone H3 (Lys4) Antibody - 300 µl $1,025.00
$922.50
ADD TO CART
9727S Tri-Methyl-Histone H3 (Lys4) Antibody - 100 µl $433.00
$389.70
ADD TO CART
9728S Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb - 100 µl $480.00
$432.00
ADD TO CART
9728T Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb - 20 µl $192.00
$172.80
ADD TO CART
9733S Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb - 100 µl $433.00
$389.70
ADD TO CART
9733T Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb - 20 µl $174.00
$156.60
ADD TO CART
9753L Di-Methyl-Histone H3 (Lys9) Antibody - 300 µl $1,025.00
$922.50
ADD TO CART
9753S Di-Methyl-Histone H3 (Lys9) Antibody - 100 µl $433.00
$389.70
ADD TO CART
9763S Tri-Methyl-Histone H3 (Lys36) Antibody - 100 µl $433.00
$389.70
ADD TO CART
9764S Phospho-Histone H3 (Thr11) Antibody - 100 µl $433.00
$389.70
ADD TO CART
9767S Phospho-Histone H3 (Thr11) (C2A6) Rabbit mAb - 100 µl $433.00
$389.70
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9927T Acetyl-Histone H3 Antibody Sampler Kit - 1 Kit $618.00
$556.20
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse, Rat, Hamster, Monkey, D. melanogaster, Zebrafish, Bovine, Dog, Pig Endogenous 17 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting

Protocols

Specificity / Sensitivity

Histone H3 (3H1) Rabbit Monoclonal Antibody detects endogenous levels of total histone H3 protein, including the Histone H3 variant CENP-A. This antibody does not cross-react with other core histones.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of human histone H3.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Histone H3 (3H1) Rabbit mAb.

Background

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

  1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
  2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
  3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
  4. Cheung, P. et al. (2000) Cell 103, 263-71.
  5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
  6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
  7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
  8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
  9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
  10. Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.
  11. Dai, J. et al. (2005) Genes Dev 19, 472-88.

Application References

  • Felder, T.K. et al. (2011) J Biol Chem 286, 42923-36. Applications: Western Blotting.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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