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Chitin Magnetic Beads

Catalog # Concentration Size List Price Quantity Your Price
E8036S 5 ml $212.00
$190.80
Catalog # Size List Price Your Price
E8036S 5 ml $212.00
$190.80
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

An affinity matrix for the small-scale isolation of target proteins fused to a chitin binding domain (CBD).

  • Chitin beads have been prepared having a magnetite core, thereby permitting the magnetic isolation of CBD-fusion proteins from cell culture supernatants
  • The matrix can be regenerated without loss of binding capacity
  • Immobilized fusion proteins can be used in subsequent experiments to capture (pull down) target proteins from crude cell lysates that interact with the immobilized fusion protein
  • Binding capacity of 2 mg chitin binding domain protein/ml bed volume
  • Quick, small-scale purification of CBD-fusion proteins enables higher throughput and efficiency
  • Removal of CBD-tag during elution typically yields highly pure, native protein

An affinity matrix for the small-scale isolation of target proteins fused to a chitin binding domain (CBD) (1). Chitin beads have been prepared having a magnetite core. This permits the magnetic isolation of CBD-fusion proteins from cell culture supernatants; after which, the matrix can be regenerated without loss of binding capacity. Immobilized fusion proteins can be used in subsequent experiments to capture target proteins from crude cell lysates that interact with the immobilized fusion protein.

IMPACT Citations

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact custom@neb.com for further information.

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Chitin Magnetic Beads E8036SVIAL 4 1 x 5 ml

Properties & Usage

Support Matrix

Approximately 50-70 μm paramagnetic microparticles

Binding Capacity

2 mg chitin binding domain protein / ml bed volume released

Can be Regenerated

Yes

Companion Products
Notes
  • Do not freeze
  • Efficiency of elution can be checked by eluting any protein that remains bound to the chitin magnetic beads with 50 µl of SDS-PAGE gel loading buffer and running 15 µl on a denaturing protein gel.
References
  • Chong, S., Mersha, F. B., Comb, D. G., Scott, M. E., Landry, D., Vence, L. M., Perler, F. B., Benner, J., Kucera, R. B., Hirvonen, C. A., Pelletier, J. J., Paulus, H. and Xu, M.-Q. (1997). Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein-splicing element.. Gene. 192, 277-281.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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