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Ph.D.™-12 Phage Display Peptide Library

Catalog # Concentration Size List Price Quantity Your Price
E8111L 50 png expmts $3,872.00
$3,484.80
Catalog # Size List Price Your Price
E8111L 50 png expmts $3,872.00
$3,484.80
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

The Ph.D.-12™ Phage Display Peptide Library contains a single tube of the Ph.D. -12 Phage Library. The Ph.D.-12 library is a combinatorial library of random 12-mer peptides fused to a minor coat protein (pIII) of M13 phage. The displayed peptide is expressed at the N-terminus of pIII. The library consists of approximately 109 electroporated (i.e., unique) sequences.

  • Ready to use complex phage library
  • Applications and methodologies are numerous and supported by over 20 years of literature citations
  • Does not require helper phage for amplification
  • Inherent link between phenotype and genotype allows screening of billions of clones in a single microtiter well or Eppendorf tube

The Ph.D.-12 Phage Display Peptide Library is based on a combinatorial library of random dodecapeptides fused to a minor coat protein (pIII) of M13 phage (1–6). The displayed peptide (12-mer) is expressed at the N-terminus of pIII, i.e., the first residue of the mature protein is the first randomized position. The peptide is followed by a short spacer (Gly-Gly-Gly-Ser) and then the wild-type pIII sequence. The library consists of approximately 109 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.

Citations for Phage Display



Panning with a pentavalent peptide library displayed on pIII.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Ph.D.™-12 Phage Display Peptide Library E8111LVIAL -20 1 x 0.5 ml 1 x 1013 pfu/ml

Properties & Usage



Companion Products
References
  • Devlin, J.J., Panganiban, L.C. and Devlin, P.E. (1990). Science. 249, 404-406.
  • Smith, G.P. and Scott, J.K. (1993). Methods Enzymol.. 217, 228-257. San Diego: Academic Press.
  • Cortese et al. (1995). Curr. Opin. Biotechnol.. 6,
  • Scott, J.K. and Smith, G.P. (1990). Science. 249, 386-390.
  • Cwirla, S.E., Peters, E.A., Barret, R.W. and Dower, W.J. (1990). Proc. Natl. Acad. Sci. USA. 87, 6378-6382.
  • Devlin, J.J., Panganiban, L.C. and Devlin, P.E. (1990). Science. 249, 404-406.
Citations
Additional Citations
  • Giraldo A.MV., Mary C., Sivanesan D., Baron C. (2015) VirB6 and VirB10 from the Brucella type IV secretion system interact via the N-terminal periplasmic domain of VirB6 FEBS Lett 589, 1883-9.PubMedID: 26071378, DOI: 10.1016/j.febslet.2015.05.051
  • Wang H., Fangxiao J., Liang H., Gu H. (2015) A Proline-Rich Domain in the Genotype 4 Hepatitis E Virus ORF3 PLoS One 10, e0133282.PubMedID: 26177202, DOI: 10.1371/journal.pone.0133282
  • Zhang L., Zhang H., Fan Z., Zhou X., Yu L., Sun H., Wu Z., Yu Y., Song B., Ma J., Tong C., Wang X., Zhu Z., Cui Y. (2015) Identification of a Conserved Linear B-Cell Epitope of Streptococcus dysgalactiae GapC Protein by Screening Phage-Displayed Random Peptide Library PLoS One 10, e0131221.PubMedID: 26121648, DOI: 10.1371/journal.pone.0131221
  • Maneewatch S, Adisakwattana P, Chaisri U, Saengjaruk P, Srimanote P, Thanongsaksrikul J, Sakolvaree Y, Poungpan P, Chaicumpa W (2014) Therapeutic epitopes of Leptospira LipL32 protein and their characteristics Protein Eng Des Sel 27(5), 135-44.PubMedID: 24760832, DOI: 10.1093/protein/gzu006
Publications
  • Subbarayal B., Schiller D., Möbs C., Pfützner W., Jahn-Schmid B., Gepp B., Breiteneder H., Vieths S., Bohle B. (2015). The diversity of Bet v 1-specific IgG4 antibodies remains mostly constant during the course of birch pollen immunotherapy J Allergy Clin Immunol.PubMedID: 26143181, DOI: 10.1016/j.jaci.2015.05.025
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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