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Histone H3.1/H4 Tetramer Human, Recombinant

Catalog # Concentration Size List Price Quantity Your Price
M2509S 10 µM 1 nmol
Please Inquire
Catalog # Size List Price Your Price
M2509S 1 nmol
Please Inquire
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Histone H3 combines with Histone H4 to form the H3/H4 tetramer. Because the histones are folded with their subunit partners, the tetramer may be a better substrate for specific enzymes and modifications.

  • Highly pure, recombinant proteins
  • Mass confirmed by ESI-TOF MS
  • Tested for exonuclease, endonuclease and protease contamination

Histone H3.1 combines with Histone H4 to form the H3/H4 tetramer. Two H2A/H2B heterodimers interact with an H3/H4 tetramer to form the histone octamer. Histone H3.1, an H3 variant that has thus far only been found in mammals, is replication dependent and is associated with gene activation and gene silencing. The tetramer is also modified by various enzymes and can act as a substrate for them. These modifications have been shown to be important in gene regulation. Because the histones are folded with their subunit partners, the tetramer may be a better substrate for specific enzymes and modifications.

Human Histone H3.1 Protein Sequence: ARTKQ TARKS TGGKA PRKQL ATKAA RKSAP ATGGV KKPHR YRPGT VALRE IRRYQ KSTEL LIRKL PFQRL VREIA QDFKT DLRFQ SSAVM ALQEA CEAYL VGLFE DTNLC AIHAK RVTIM PKDIQ LARRI RGERA (Genbank accession number: AAN10051)

Human Histone H4 Protein Sequence: SGRGK GGKGL GKGGA KRHRK VLRDN IQGIT KPAIR RLARR GGVKR ISGLI YEETR GVLKV FLENV IRDAV TYTEH AKRKT VTAMD VVYAL KRQGR TLYGF GG (Genbank accession number: AAM83108)

SDS-PAGE analysis of Histone H3.1/H4 Tetramer Human, Recombinant
SDS-PAGE analysis of Histone H3.1/H4 Tetramer Human, Recombinant
Lane 1: NEB Protein Ladder (NEB #P7703 )
Lane 2: Histone H3.1 (NEB #M2503)
Lane 3: Histone H4 (NEB #M2504)
Lane 4–6: 2.0, 4.0, 8.0 µg Histone H3.1/H4 Tetramer
Product Source
Purified H3.1 and H4 (NEB #M2503 and NEB #M2504) were denatured, refolded and the Histone H3/H4 Tetramer was purified by gel filtration.

Properties & Usage

Storage Buffer

20 mM Tris-HCl
2 M NaCl
1 mM DTT
1 mM EDTA
pH 8 @ 25°C

Molecular Weight

Theoretical: 53018 daltons


Companion Products
Notes
  • 10 μM, 0.53 mg/ml is calculated using the molar extinction coefficient for Histone H3.1/H4 Tetramer (21,360) and its absorbance at 280 nm (5,6)
  • To use as a substrate in an enzyme modification assay or other salt sensitive protocol, use 1 to 2 µl of the tetramer in a minimum 20 µl reaction so that the salt concentration in the reaction is ≤ 200 mM.
  • Because of high salt, the solution does not always remain frozen.
References
  • Kornberg, R.D. (1977). Annu. Rev. Biochem,. 46, 931-954.
  • van Holde, K.E. (1989). Chromatin,. 1-497,
  • Hake, S.B. et al. (2006). J. Biol. Chem,. 281, 559-568.
  • Mersha, F unpublished observation.
  • Pace, C.N. et al. (1995). Protein Science. 4, 2411-2423.
  • Gill, S.C. and von Hippel, P.H. (1989). Anal. Biochem. 182, 319-326.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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