Product Class: Kit

Cloning Competent E.coli Sampler
Item# Description List Price Web Price Qty
C1010S NEB Cloning Competent E.coli Sampler - 2 x 50 ul $170.00
$153.00
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

    Product Introduction

    Not sure which cloning strain to choose? The NEB Cloning Competent E.coli Sampler allows you to sample 4 of our popular chemically competent strains. The Sampler includes:

    • NEB 5-alpha: Our best-selling strain for basic cloning; Derivative of the popular DH5α. It is T1 phage resistant and endA deficient for high-quality plasmid preparations.
    • NEB 10-beta: derivative of the popular DH10B. It is T1 phage resistant and endonuclease I (endA1) deficient for high-quality plasmid preparations.
    • NEB Stable: Ideal for isolation of plasmid clones containing repeat elements and unstable inserts. Useful for isolating and propagating retroviral/lentiviral clones
    • NEB Turbo: Features fast colony growth (6.5 hours) and tight expression control (lacIq). Isolate DNA after only 4 hours of growth

    Product Information

    Description

    Highlights

    • Transformation efficiencies of 1-3 x 109 cfu/μg pUC19 DNA
    • Resistance to phage T1 (fhuA2)
    • Outgrowth medium and control plasmid included
    • Free of animal products
    • Value pricing
    • No dry ice charges
    A sample pack of four cloning strains of E. Coli suitable for high efficiency transformation.

    Kit Components

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    SOC Outgrowth Medium 4
    NEB® 10-beta/Stable Outgrowth Medium 4 1X
    NEB® Turbo Competent E. coli (High Efficiency) -80
    NEB® 5-alpha Competent E. coli (High Efficiency) -80
    NEB® 10-beta Competent E. coli (High Efficiency) -80
    NEB® Stable Competent E. coli (High Efficiency) -80
    pUC19 Vector -20 0.05 ng/µl
    Product Categories:
    Competent Cells Products,
    Cloning Competent Cell Strains Products
    Applications:
    Transformation

    Properties & Usage

    Product Notes

    1. CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.

    2. STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.

    Protocols, Manuals & Usage

    Usage & Guidelines

    • Chemical Transformation Tips

    Tools & Resources

    Selection Charts

    • Competent Cell Product Comparison
    • Competent Cell Selection Guide
    • Strain Properties

    Faqs & Troubleshooting

    FAQs

    1. Why did Synthetic Biologist Chris Voigt of MIT choose NEB 10-beta for DNA assembly and cloning?

    Troubleshooting

    • Troubleshooting Transformation Reactions

    Quality & Safety

    Quality Assurance Statement

    Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    Certificate Of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Safety DataSheets

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Legal and Disclaimers

    This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

    While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

    For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

    This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.