Product Class: Other

RNase If
icon_recomb NEBuffer 3 icon_incTemp37 icon_HI_70
Item# Description List Price Web Price Qty
M0243L RNase I - 25,000 units $384.00
$345.60
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M0243S RNase I - 5,000 units $95.00
$85.50
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Product Introduction

RNase If eliminates single-stranded RNA from DNA and protein preparations 

  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer
  • Tested for the absence of DNA endonucleases and exonucleases 

Product Information

Description

Ribonuclease If (RNase If) is an RNA endonuclease which will cleave at all RNA dinucleotide bonds leaving a 5´ hydroxyl
and 2´,3´ cyclic monophosphate (1). It has a preference for single-stranded RNA over double-stranded RNA. RNase If is a recombinant protein fusion of RNase I (from E. coli) and maltose-binding protein. It has identical activity to RNase I.

Product Source

An E. coli strain containing a genetic fusion of the E. coli RNase I gene (2nd) and the gene coding for maltose binding protein (MBP).

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C) Concentration
NEBuffer™ 3 -20 10 X
Product Categories:
RNases Products
Applications:
RNA Modification

Advantages and Features

Application Features

  • Degradation of single-stranded RNA to mono-, di- and trinucleotide (3)
  • Used in ribonuclease protection assays

Properties & Usage

    Unit Definition

    One unit is defined as the amount of enzyme required to fully digest 1 picomole of synthetic ssRNA 33-mer in a total reaction volume of 10 µl in 15 minutes in 1X NEBuffer 3 as visualized on a 20% acrylamide gel.

    Reaction Conditions

    1X NEBuffer™ 3
    Incubate at 37°C

    1X NEBuffer™ 3
    100 mM NaCl
    50 mM Tris-HCl
    10 mM MgCl2
    1 mM DTT
    (pH 7.9 @ 25°C)

    Storage Temperature

    -20°C

    Storage Conditions

    10 mM Tris-HCl
    100 mM NaCl
    1 mM DTT
    0.5 mM EDTA
    50% Glycerol
    pH 8.0 @ 25°C

    Heat Inactivation

    70°C for 20 min

    Product Notes

    1. RNase If will not degrade DNA. It has a preference for single-stranded RNA over double-stranded RNA.

    References

    1. Spahr, P.F. and Hollingworth, B.R. (1961). J. Biol. Chem.. 236, 823-831.
    2. Meador, J. III and Kennell, D. (1990). Gene. 95, 1-7.
    3. Meador, J. III, Cannon, B., Cannistraro, V.J. and Kennell, D. (1990). Eur. J. Biochem.. 187, 549-553.

    Protocols, Manuals & Usage

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)

    Quality, Safety & Legal

    Quality Assurance Statement

    Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    Specifications

    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    Certificate Of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Safety DataSheets

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Legal and Disclaimers

    This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

    While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

    For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

    This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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