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AMV Reverse Transcriptase

Catalog # Concentration Size List Price Quantity Your Price
M0277L 10000 units/ml 1000 units $510.00
$459.00
M0277S 10000 units/ml 200 units $128.00
$115.20
Catalog # Size List Price Your Price
M0277L 1000 units $510.00
$459.00
M0277S 200 units $128.00
$115.20
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • DNA synthesis from either RNA or ssDNA
  • Ideal for RT-PCR, cDNA synthesis, and RNA sequencing
  • 10X reaction buffer creates optimal reaction conditions for robust first and second-strand cDNA synthesis
Featured Videos
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  • Monarch_MiniprepProtocol_VideoThumb
    Avoiding RNase Contamination
Avian Myeloblastosis Vi­rus (AMV) Reverse Transcriptase is an RNA-directed DNA polymerase. This en­zyme can synthesize a complementary DNA strand initiating from a primer using RNA (cDNA synthesis) or single-stranded DNA as a template (1-3).
Product Source
Insect cells (Sf21) infected with baculovirus containing the pol gene of AMV.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  AMV Reverse Transcriptase M0277SVIAL -20 1 x 0.02 ml 10,000 units/ml
  AMV Reverse Transcriptase Reaction Buffer B0277AVIAL -20 1 x 1.5 ml 10 X
  AMV Reverse Transcriptase M0277LVIAL -20 1 x 0.1 ml 10,000 units/ml
  AMV Reverse Transcriptase Reaction Buffer B0277AVIAL -20 1 x 1.5 ml 10 X
Application Features
  • cDNA Synthesis
  • RNA Sequencing
  • RT-PCR

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into an acid-insoluble form in 10 minutes at 37°C using poly(rA)-oligo(dT) as template primer.

Reaction Conditions

1X AMV Reverse Transcriptase Reaction Buffer
Incubate at 42°C

1X AMV Reverse Transcriptase Reaction Buffer
50 mM Tris-acetate
75 mM Potassium Acetate
8 mM Magnesium Acetate
10 mM DTT
(pH 8.3 @ 25°C)

Storage Buffer

200 mM potassium phosphate
2 mM DTT
50% Glycerol
0.2% Triton® X-100
pH 7.2 @ 25°C

Heat Inactivation

85°C for 5 minutes

Unit Assay Conditions

75 mM potassium acetate, 50 mM Tris-HCl (pH 8.3) 8 mM magnesium acetate, 0.5 mM [3H]-dTTP, 0.2 mM poly(rA)•oligo(dT)12–18 and 10 mM DTT.

Companion Products
Notes
  • Reaction Conditions: Incubate at 37-52°C.
  • The yield and size of cDNA transcript increases with increasing amounts of RT.
  • Storage: Once thawed, store at -20°C. Repeated freeze thaw cycles will inactivate the enzyme. Aliquots can be stored for longer periods at -70°C.
References
  • Krug, M.S. et al. (1987). Meth.Enzymol. 152,
  • Sambrook.J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A LaboratoryManual. (2nd ed.), 8-64. Cold Spring Harbor Laboratory Press.
  • Kacian, D.L. (1977). Meth.Virol.. 6, 143.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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