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T4 Gene 32 Protein

Catalog # Concentration Size List Price Quantity Your Price
M0300L 10 mg/ml 500 µg $522.00
$469.80
M0300S 10 mg/ml 100 µg $128.00
$115.20
Catalog # Size List Price Your Price
M0300L 500 µg $522.00
$469.80
M0300S 100 µg $128.00
$115.20
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

T4 Gene 32 Protein is a single-stranded DNA binding protein required for bacteriophage T4 replication and repair.

  • Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure
  • Increase yield and processivity of reverse transcriptase during RT-PCR
  • Increase yield and specificity of PCR products from soil samples
T4 Gene 32 Protein is a single-stranded DNA (ssDNA) binding protein required for bacteriophage T4 replication and repair (1-2). It cooperatively binds to and stablizes transiently formed regions of ssDNA and plays an important structural role during T4 phage replication (3). It also has been used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structures (4-5). Recently, it has been shown to improve restriction enzyme digestion (6), improve the yield and efficiency of reverse transcription (RT) reactions during RT-PCR (7-9), enhance T4 DNA polymerase activity (10-11), as well as increase the yield of PCR products (12).
Product Source
An E. coli strain carrying a plasmid that overexpresses the gene 32 protein of T4 phage
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  T4 Gene 32 Protein M0300SVIAL -20 1 x 0.01 ml 10 mg/ml
  NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X
  T4 Gene 32 Protein M0300LVIAL -20 1 x 0.05 ml 10 mg/ml
  NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X
Application Features
  • Stabilization and marking of ssDNA structures (4-5)
  • Increase yield and processivity of RT during RT-PCR (7-9)
  • Increase yield and specificity of PCR products from soil samples (10-12)

Properties & Usage

Reaction Conditions

1X NEBuffer™ 4
Incubate at 37°C

1X NEBuffer™ 4
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
1 mM DTT
(pH 7.9 @ 25°C)

Storage Buffer

20 mM Tris-HCl
100 mM NaCl
0.5 mM DTT
1 mM EDTA
50% Glycerol
pH 8 @ 25°C

Heat Inactivation

65°C for 20 minutes

Molecular Weight

Theoretical: 33506 daltons


Materials Sold Separately
References
  • Alberts, B. and Frey, L. (1970). Nature. 227, 1313-1318.
  • Huberman, J.A. and Kornberg, A. (1971). J. Mol. Biol. 62, 39-52.
  • Topal, M.D. and Sinha, N.K. (1983). J. Biol. Chem. 258, 12274-12279.
  • Schwartz, K. et al. (1990). Nucl. Acids Res. 18, 1079.
  • Kornberg, A. and Baker, T. DNA Replication. (2nd ed.), 328-332.
  • Bittner, M. et al. (1979). J. Biol. Chem. 254, 9565-9572.
  • Delius, H. et al. (1972). J. Mol. Biol.. 67, 341-350.
  • Wu, M. and Davidson, N. (1975). Proc. Natl. Acad. Sci. USA. 72, 4506.
  • Dombroski, D.F. and Morgan, A.R. (1985). J. Biol. Chem. 260, 415-447.
  • Villalva, C. et al. (2001). Biotechniques. 31, 81-86.
  • Baugh, L.R. et al. (2001). Nucl. Acids Res. 29, e29.
  • Jeffries, D. and Farquharson, C. (2002). Anal Biochem. 308, 192-194.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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